Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 639543..640270 Replicon chromosome
Accession NZ_CP080253
Organism Escherichia coli strain PC6

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KZX77_RS03120 Protein ID WP_000550189.1
Coordinates 639543..639857 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KZX77_RS03125 Protein ID WP_000560266.1
Coordinates 639854..640270 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX77_RS03100 (635701) 635701..636687 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
KZX77_RS03105 (636766) 636766..637458 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KZX77_RS03110 (637535) 637535..637987 - 453 WP_001336107.1 M48 family metallopeptidase -
KZX77_RS03115 (638123) 638123..639259 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KZX77_RS03120 (639543) 639543..639857 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KZX77_RS03125 (639854) 639854..640270 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KZX77_RS03130 (640315) 640315..642333 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KZX77_RS03135 (642759) 642759..645110 - 2352 WP_061093122.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T211452 WP_000550189.1 NZ_CP080253:639543-639857 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T211452 NZ_CP104866:2251115-2251369 [Pseudomonas aeruginosa]
GTGAAGCTGATCTGGTCGGAACAAGCCTGGGACGATTATCTGTACTGGCAGGACACGGATAAGCGTATGGTGAAAAAGGT
CAACGAGTTGATCAAGGATGCCTGCCGTACCCCGTTCGAGGGCAAGGGCAAGCCGGAGCCGCTTCGCCACAACCTGGCGG
GCTTCTGGTCGAGACGGATCACGGCCGAGCATCGGTTTGTGTATTCGGTGACGGATGACGCGCTGCTGATTGCAGCCTGC
CGTTACCACTATTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT211452 WP_000560266.1 NZ_CP080253:639854-640270 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT211452 NZ_CP104866:2250867-2251118 [Pseudomonas aeruginosa]
ATGCGCACGATCAGTTACACCGAAGCCCGGCAGAACCTGTCGGCCACCATGATGAAGACCGTGGAGGATCGCGCCCCGAT
CCTGATTACCCGGCAGAACGGCGAAGCCTGCGTGTTGATGTCCCTGGAAGAGTTCAACGCGCTGGAGGAAACGGCTTATC
TGCTGCGCTCCCCGGCCAATGCCCGCCGCCTGATGGATTCGATTGACAGCCTCAAGCAAGGCCAGGGCGTCGAGCGGGAT
TTGGCCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References