Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 596197..596996 Replicon chromosome
Accession NZ_CP080253
Organism Escherichia coli strain PC6

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KZX77_RS02895 Protein ID WP_000347273.1
Coordinates 596197..596661 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KZX77_RS02900 Protein ID WP_001307405.1
Coordinates 596661..596996 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX77_RS02865 (591198) 591198..591632 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KZX77_RS02870 (591650) 591650..592528 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KZX77_RS02875 (592518) 592518..593297 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZX77_RS02880 (593308) 593308..593781 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZX77_RS02885 (593804) 593804..595084 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZX77_RS02890 (595333) 595333..596142 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KZX77_RS02895 (596197) 596197..596661 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZX77_RS02900 (596661) 596661..596996 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZX77_RS02905 (597145) 597145..598716 - 1572 WP_001273753.1 galactarate dehydratase -
KZX77_RS02910 (599091) 599091..600425 + 1335 WP_061093123.1 galactarate/glucarate/glycerate transporter GarP -
KZX77_RS02915 (600441) 600441..601211 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T211451 WP_000347273.1 NZ_CP080253:c596661-596197 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T211451 NZ_CP104866:2250867-2251118 [Pseudomonas aeruginosa]
ATGCGCACGATCAGTTACACCGAAGCCCGGCAGAACCTGTCGGCCACCATGATGAAGACCGTGGAGGATCGCGCCCCGAT
CCTGATTACCCGGCAGAACGGCGAAGCCTGCGTGTTGATGTCCCTGGAAGAGTTCAACGCGCTGGAGGAAACGGCTTATC
TGCTGCGCTCCCCGGCCAATGCCCGCCGCCTGATGGATTCGATTGACAGCCTCAAGCAAGGCCAGGGCGTCGAGCGGGAT
TTGGCCGAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT211451 WP_001307405.1 NZ_CP080253:c596996-596661 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT211451 NZ_CP104866:2251115-2251369 [Pseudomonas aeruginosa]
GTGAAGCTGATCTGGTCGGAACAAGCCTGGGACGATTATCTGTACTGGCAGGACACGGATAAGCGTATGGTGAAAAAGGT
CAACGAGTTGATCAAGGATGCCTGCCGTACCCCGTTCGAGGGCAAGGGCAAGCCGGAGCCGCTTCGCCACAACCTGGCGG
GCTTCTGGTCGAGACGGATCACGGCCGAGCATCGGTTTGTGTATTCGGTGACGGATGACGCGCTGCTGATTGCAGCCTGC
CGTTACCACTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References