Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3571255..3571949 Replicon chromosome
Accession NZ_CP080247
Organism Escherichia sp. TM-G17TGC

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag KY274_RS17455 Protein ID WP_001263489.1
Coordinates 3571255..3571653 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag KY274_RS17460 Protein ID WP_000554758.1
Coordinates 3571656..3571949 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3566843) 3566843..3566923 - 81 NuclAT_12 - -
- (3566843) 3566843..3566923 - 81 NuclAT_12 - -
- (3566843) 3566843..3566923 - 81 NuclAT_12 - -
- (3566843) 3566843..3566923 - 81 NuclAT_12 - -
KY274_RS17430 (3567519) 3567519..3567977 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
KY274_RS17435 (3568238) 3568238..3569695 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
KY274_RS17440 (3569752) 3569752..3570273 - 522 Protein_3413 peptide chain release factor H -
KY274_RS17445 (3570269) 3570269..3570475 - 207 Protein_3414 RtcB family protein -
KY274_RS17450 (3570793) 3570793..3571245 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
KY274_RS17455 (3571255) 3571255..3571653 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KY274_RS17460 (3571656) 3571656..3571949 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KY274_RS17465 (3572001) 3572001..3573056 - 1056 WP_001226164.1 DNA polymerase IV -
KY274_RS17470 (3573127) 3573127..3573912 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KY274_RS17475 (3573884) 3573884..3575596 + 1713 Protein_3420 flagellar biosynthesis protein FlhA -
KY274_RS17480 (3575820) 3575820..3576317 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T211399 WP_001263489.1 NZ_CP080247:c3571653-3571255 [Escherichia sp. TM-G17TGC]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T211399 NZ_CP104851:c4336861-4336688 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT211399 WP_000554758.1 NZ_CP080247:c3571949-3571656 [Escherichia sp. TM-G17TGC]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT211399 NZ_CP104851:c4337185-4336889 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References