Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1912282..1912784 Replicon chromosome
Accession NZ_CP080246
Organism Escherichia coli strain SYNB1934

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag KZX95_RS08985 Protein ID WP_000767822.1
Coordinates 1912530..1912784 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag KZX95_RS08980 Protein ID WP_001259255.1
Coordinates 1912282..1912533 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX95_RS08955 (1907460) 1907460..1908527 - 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
KZX95_RS08960 (1908527) 1908527..1909597 - 1071 WP_000108995.1 histidinol-phosphate transaminase -
KZX95_RS08965 (1909594) 1909594..1910898 - 1305 WP_001297912.1 histidinol dehydrogenase -
KZX95_RS08970 (1910904) 1910904..1911803 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
KZX95_RS08975 (1911949) 1911949..1911999 - 51 WP_001364200.1 his operon leader peptide -
KZX95_RS08980 (1912282) 1912282..1912533 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
KZX95_RS08985 (1912530) 1912530..1912784 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
KZX95_RS08990 (1912867) 1912867..1913691 + 825 WP_000754733.1 SDR family oxidoreductase -
KZX95_RS08995 (1913737) 1913737..1914666 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
KZX95_RS09000 (1914881) 1914881..1914943 + 63 WP_010723108.1 membrane protein YoeI -
KZX95_RS09005 (1914933) 1914933..1916291 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T211353 WP_000767822.1 NZ_CP080246:1912530-1912784 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T211353 NZ_CP104847:c30801-30421 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT211353 WP_001259255.1 NZ_CP080246:1912282-1912533 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT211353 NZ_CP104847:c31022-30801 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References