Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1206673..1207400 Replicon chromosome
Accession NZ_CP080246
Organism Escherichia coli strain SYNB1934

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag KZX95_RS05780 Protein ID WP_000547555.1
Coordinates 1206673..1206984 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag KZX95_RS05785 Protein ID WP_000126293.1
Coordinates 1206981..1207400 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX95_RS05750 (1201814) 1201814..1203523 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
KZX95_RS05755 (1203533) 1203533..1204075 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
KZX95_RS05760 (1204075) 1204075..1204842 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
KZX95_RS05765 (1204839) 1204839..1205249 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
KZX95_RS05770 (1205242) 1205242..1205712 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
KZX95_RS05775 (1205755) 1205755..1206510 + 756 WP_001305268.1 hypothetical protein -
KZX95_RS05780 (1206673) 1206673..1206984 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
KZX95_RS05785 (1206981) 1206981..1207400 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
KZX95_RS05790 (1207621) 1207621..1207920 - 300 WP_001704025.1 DUF4259 domain-containing protein -
KZX95_RS05795 (1208305) 1208305..1208832 + 528 WP_001078777.1 electron transport protein HydN -
KZX95_RS05800 (1208985) 1208985..1211237 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T211352 WP_000547555.1 NZ_CP080246:1206673-1206984 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T211352 NZ_CP104847:5334-5468 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT211352 WP_000126293.1 NZ_CP080246:1206981-1207400 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT211352 NZ_CP104847:c5289-5229 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References