Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4300822..4301516 Replicon chromosome
Accession NZ_CP080235
Organism Escherichia coli O139:H1 strain W13-16

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag KZW92_RS21020 Protein ID WP_001521903.1
Coordinates 4301118..4301516 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag KZW92_RS21015 Protein ID WP_000554755.1
Coordinates 4300822..4301115 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW92_RS20990 (4296443) 4296443..4296799 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
KZW92_RS20995 (4296792) 4296792..4297070 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KZW92_RS21000 (4297175) 4297175..4298887 - 1713 Protein_4113 flagellar biosynthesis protein FlhA -
KZW92_RS21005 (4298859) 4298859..4299644 + 786 WP_074150236.1 putative lateral flagellar export/assembly protein LafU -
KZW92_RS21010 (4299715) 4299715..4300770 + 1056 WP_001226166.1 DNA polymerase IV -
KZW92_RS21015 (4300822) 4300822..4301115 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KZW92_RS21020 (4301118) 4301118..4301516 + 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KZW92_RS21025 (4301526) 4301526..4301978 + 453 WP_001059858.1 GNAT family N-acetyltransferase -
KZW92_RS21030 (4302297) 4302297..4303436 + 1140 WP_063085369.1 RNA ligase RtcB family protein -
KZW92_RS21035 (4303433) 4303433..4304047 + 615 WP_000602129.1 peptide chain release factor H -
KZW92_RS21040 (4304109) 4304109..4304618 + 510 WP_063085380.1 metal-dependent hydrolase -
KZW92_RS21045 (4304635) 4304635..4304916 - 282 WP_077881290.1 hypothetical protein -
KZW92_RS21050 (4304925) 4304925..4306382 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T211332 WP_001521903.1 NZ_CP080235:4301118-4301516 [Escherichia coli O139:H1]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T211332 NZ_CP104846:186695-186802 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT211332 WP_000554755.1 NZ_CP080235:4300822-4301115 [Escherichia coli O139:H1]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT211332 NZ_CP104846:c186638-186581 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References