Detailed information of TA system TA211328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3399974..3400576
• Replicon: chromosome
• Accession: NZ_CP080235
• Organism: Escherichia coli O139:H1 strain W13-16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KZW92_RS16635
• Protein ID: WP_000897305.1
• Coordinates: 3399974..3400285 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZW92_RS16640
• Protein ID: WP_000356395.1
• Coordinates: 3400286..3400576 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW92_RS16615 (3395015)	3395015..3395452	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KZW92_RS16620 (3395497)	3395497..3396438	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KZW92_RS25805 (3396854)	3396854..3397741	+	888	Protein_3261	hypothetical protein	-
KZW92_RS25810 (3397754)	3397754..3398668	+	915	WP_225102955.1	transposase	-
KZW92_RS16630 (3398837)	3398837..3399745	-	909	WP_001306651.1	alpha/beta hydrolase	-
KZW92_RS16635 (3399974)	3399974..3400285	+	312	WP_000897305.1	hypothetical protein	Toxin
KZW92_RS16640 (3400286)	3400286..3400576	+	291	WP_000356395.1	NadS family protein	Antitoxin
KZW92_RS16645 (3400661)	3400661..3400873	+	213	WP_000197769.1	hypothetical protein	-
KZW92_RS16650 (3400935)	3400935..3401213	+	279	WP_001306650.1	hypothetical protein	-
KZW92_RS16655 (3401632)	3401632..3401850	+	219	WP_001295676.1	CopG family transcriptional regulator	-
KZW92_RS16660 (3402069)	3402069..3402311	+	243	WP_001306649.1	protein YiiF	-
KZW92_RS16665 (3402493)	3402493..3403422	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KZW92_RS16670 (3403419)	3403419..3404054	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KZW92_RS16675 (3404051)	3404051..3404953	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T211328 WP_000897305.1 NZ_CP080235:3399974-3400285 [Escherichia coli O139:H1]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T211328 NZ_CP104844:c20987-20466 [Escherichia coli]
GTGAGCAATACAACGATAGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCCTTTTTAGCCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGTGCTTATGTGCTTTGCACTCAAGAAG
AAAGGCCAAAAGTGTTAGGATATTACACTTTGTCAGGTAGTTGTTTTGAAAAGGAGTCCTTACCTTCAAGAAGCAAACAA
AAGAAGGTTCCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT211328 WP_000356395.1 NZ_CP080235:3400286-3400576 [Escherichia coli O139:H1]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT211328 NZ_CP104844:c21264-20995 [Escherichia coli]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TAGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCTGATCGTCTGAAAAGCATGGAGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7