Detailed information of TA system TA211327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2567159..2567958
• Replicon: chromosome
• Accession: NZ_CP080235
• Organism: Escherichia coli O139:H1 strain W13-16

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A3A6SBL4
• Locus tag: KZW92_RS12660
• Protein ID: WP_062875446.1
• Coordinates: 2567494..2567958 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: KZW92_RS12655
• Protein ID: WP_001307405.1
• Coordinates: 2567159..2567494 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW92_RS12640 (2562939)	2562939..2563714	-	776	Protein_2488	2-dehydro-3-deoxyglucarate aldolase	-
KZW92_RS12645 (2563730)	2563730..2565064	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
KZW92_RS12650 (2565439)	2565439..2567010	+	1572	WP_001273756.1	galactarate dehydratase	-
KZW92_RS12655 (2567159)	2567159..2567494	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
KZW92_RS12660 (2567494)	2567494..2567958	+	465	WP_062875446.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
KZW92_RS12665 (2568013)	2568013..2568822	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
KZW92_RS12670 (2569071)	2569071..2570351	+	1281	WP_063086249.1	tagatose-bisphosphate aldolase subunit KbaZ	-
KZW92_RS12675 (2570374)	2570374..2570847	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
KZW92_RS12680 (2570858)	2570858..2571637	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
KZW92_RS12685 (2571627)	2571627..2572505	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
KZW92_RS12690 (2572523)	2572523..2572957	+	435	WP_063086247.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2557791..2567958	10167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17844.22 Da
• Isoelectric Point: 9.4942

>T211327 WP_062875446.1 NZ_CP080235:2567494-2567958 [Escherichia coli O139:H1]
MDFPQRVNSWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETD

Nucleotide

• Length: 465 bp

>T211327 NZ_CP104843:c4858774-4858463 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT211327 WP_001307405.1 NZ_CP080235:2567159-2567494 [Escherichia coli O139:H1]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT211327 NZ_CP104843:c4858462-4858172 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A3A6SBL4

Antitoxin

• AlphaFold DB: A0A0E0XVC7