Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4403816..4404615 Replicon chromosome
Accession NZ_CP080233
Organism Escherichia coli O139:H1 strain P15-25

Toxin (Protein)


Gene name yhaV Uniprot ID A0A3A6SBL4
Locus tag KZW90_RS21455 Protein ID WP_062875446.1
Coordinates 4404151..4404615 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KZW90_RS21450 Protein ID WP_001307405.1
Coordinates 4403816..4404151 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW90_RS21435 (4399596) 4399596..4400371 - 776 Protein_4200 2-dehydro-3-deoxyglucarate aldolase -
KZW90_RS21440 (4400387) 4400387..4401721 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KZW90_RS21445 (4402096) 4402096..4403667 + 1572 WP_001273756.1 galactarate dehydratase -
KZW90_RS21450 (4403816) 4403816..4404151 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZW90_RS21455 (4404151) 4404151..4404615 + 465 WP_062875446.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZW90_RS21460 (4404670) 4404670..4405479 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
KZW90_RS21465 (4405728) 4405728..4407008 + 1281 WP_063086249.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZW90_RS21470 (4407031) 4407031..4407504 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZW90_RS21475 (4407515) 4407515..4408294 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZW90_RS21480 (4408284) 4408284..4409162 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KZW90_RS21485 (4409180) 4409180..4409614 + 435 WP_063086247.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4394448..4404615 10167


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17844.22 Da        Isoelectric Point: 9.4942

>T211307 WP_062875446.1 NZ_CP080233:4404151-4404615 [Escherichia coli O139:H1]
MDFPQRVNSWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETD

Download         Length: 465 bp

>T211307 NZ_CP104843:253728-253835 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT211307 WP_001307405.1 NZ_CP080233:4403816-4404151 [Escherichia coli O139:H1]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT211307 NZ_CP104843:c253671-253616 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A6SBL4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References