Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3909527..3910254 Replicon chromosome
Accession NZ_CP080233
Organism Escherichia coli O139:H1 strain P15-25

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag KZW90_RS19145 Protein ID WP_000547555.1
Coordinates 3909943..3910254 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KZW90_RS19140 Protein ID WP_000126294.1
Coordinates 3909527..3909946 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW90_RS19120 (3904612) 3904612..3905139 - 528 WP_001078777.1 electron transport protein HydN -
KZW90_RS19125 (3905288) 3905288..3906298 - 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
KZW90_RS19130 (3906558) 3906558..3908015 + 1458 WP_001107888.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
KZW90_RS19135 (3908024) 3908024..3909448 + 1425 WP_063086210.1 6-phospho-beta-glucosidase AscB -
KZW90_RS19140 (3909527) 3909527..3909946 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
KZW90_RS19145 (3909943) 3909943..3910254 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
KZW90_RS19150 (3910421) 3910421..3910891 - 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
KZW90_RS19155 (3910884) 3910884..3911294 - 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
KZW90_RS19160 (3911291) 3911291..3912058 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
KZW90_RS19165 (3912058) 3912058..3912600 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
KZW90_RS19170 (3912610) 3912610..3914319 - 1710 WP_063086195.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T211305 WP_000547555.1 NZ_CP080233:c3910254-3909943 [Escherichia coli O139:H1]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T211305 NZ_CP104843:252762-252869 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT211305 WP_000126294.1 NZ_CP080233:c3909946-3909527 [Escherichia coli O139:H1]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT211305 NZ_CP104843:c252713-252650 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References