Detailed information of TA system TA211284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1066347..1067041
• Replicon: chromosome
• Accession: NZ_CP080233
• Organism: Escherichia coli O139:H1 strain P15-25

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: KZW90_RS05125
• Protein ID: WP_001521903.1
• Coordinates: 1066643..1067041 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: KZW90_RS05120
• Protein ID: WP_000554755.1
• Coordinates: 1066347..1066640 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW90_RS05095 (1061968)	1061968..1062324	-	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
KZW90_RS05100 (1062317)	1062317..1062595	-	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
KZW90_RS05105 (1062700)	1062700..1064412	-	1713	Protein_984	flagellar biosynthesis protein FlhA	-
KZW90_RS05110 (1064384)	1064384..1065169	+	786	WP_074150236.1	putative lateral flagellar export/assembly protein LafU	-
KZW90_RS05115 (1065240)	1065240..1066295	+	1056	WP_001226166.1	DNA polymerase IV	-
KZW90_RS05120 (1066347)	1066347..1066640	+	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KZW90_RS05125 (1066643)	1066643..1067041	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KZW90_RS05130 (1067051)	1067051..1067503	+	453	WP_001059858.1	GNAT family N-acetyltransferase	-
KZW90_RS05135 (1067822)	1067822..1068961	+	1140	WP_063085369.1	RNA ligase RtcB family protein	-
KZW90_RS05140 (1068958)	1068958..1069572	+	615	WP_000602129.1	peptide chain release factor H	-
KZW90_RS05145 (1069634)	1069634..1070143	+	510	WP_063085380.1	metal-dependent hydrolase	-
KZW90_RS05150 (1070160)	1070160..1070441	-	282	WP_077881290.1	hypothetical protein	-
KZW90_RS05155 (1070450)	1070450..1071907	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T211284 WP_001521903.1 NZ_CP080233:1066643-1067041 [Escherichia coli O139:H1]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T211284 NZ_CP104835:197629-197907 [Achromobacter xylosoxidans]
ATGCTCGATGTATCCTGGCTTCAAACCGCCGCAGACGATCTGGCGGGAATCATCTCCTACATCGCCGAGCGTAGTCCGCA
GGCGGCACGCAACCTCCGGCAACGCATCGACAATGCCGTGCTGTCCCTGGCGCGGCACCCTTGCCGATATCGGGCCGGCC
GCGTCTCTGGCACGCGCGAATGCGTCGTACACCCAAATTACGTGGTGGTCTACCGGGTGACCGCGTTGGCGATCGAGGTC
GTCAACATCGTGCACGCCCGCCGGGAATATCCCCCCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT211284 WP_000554755.1 NZ_CP080233:1066347-1066640 [Escherichia coli O139:H1]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT211284 NZ_CP104835:197354-197560 [Achromobacter xylosoxidans]
ATGGGCCCAATCCCTTCCTCCATCCTCTCGGAATTCGAGACCGAAGAACAGGCGGTCAGCTACGACCGCTGGTTCCGCGC
GAAGGTGCAAGCCAGCCTGGACGATCCGCGTCCCGCCGTTTCGCATCAAAGGGTGATGGCCCGCATCGATGCCATTATCG
AAACGGCGTGCCGTCGTCAGCTGGATCCGCAAGACGGAGAAAGCTGA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: A0A829G9H5