Detailed information of TA system TA211280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 206895..207497
• Replicon: chromosome
• Accession: NZ_CP080233
• Organism: Escherichia coli O139:H1 strain P15-25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KZW90_RS00965
• Protein ID: WP_000897305.1
• Coordinates: 206895..207206 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZW90_RS00970
• Protein ID: WP_000356395.1
• Coordinates: 207207..207497 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW90_RS00945 (201936)	201936..202373	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KZW90_RS00950 (202418)	202418..203359	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KZW90_RS24925 (203775)	203775..204662	+	888	Protein_177	hypothetical protein	-
KZW90_RS24930 (204675)	204675..205589	+	915	WP_225102955.1	transposase	-
KZW90_RS00960 (205758)	205758..206666	-	909	WP_001306651.1	alpha/beta hydrolase	-
KZW90_RS00965 (206895)	206895..207206	+	312	WP_000897305.1	hypothetical protein	Toxin
KZW90_RS00970 (207207)	207207..207497	+	291	WP_000356395.1	NadS family protein	Antitoxin
KZW90_RS00975 (207582)	207582..207794	+	213	WP_000197769.1	hypothetical protein	-
KZW90_RS00980 (207856)	207856..208134	+	279	WP_001306650.1	hypothetical protein	-
KZW90_RS00985 (208553)	208553..208771	+	219	WP_001295676.1	CopG family transcriptional regulator	-
KZW90_RS00990 (208990)	208990..209232	+	243	WP_001306649.1	protein YiiF	-
KZW90_RS00995 (209414)	209414..210343	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KZW90_RS01000 (210340)	210340..210975	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KZW90_RS01005 (210972)	210972..211874	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T211280 WP_000897305.1 NZ_CP080233:206895-207206 [Escherichia coli O139:H1]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T211280 NZ_CP080233:206895-207206 [Escherichia coli O139:H1]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT211280 WP_000356395.1 NZ_CP080233:207207-207497 [Escherichia coli O139:H1]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT211280 NZ_CP080233:207207-207497 [Escherichia coli O139:H1]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7