Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 37679..38325 Replicon plasmid pP136-7
Accession NZ_CP080230
Organism Escherichia coli O141:H4 strain P13-6

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag KZW89_RS28575 Protein ID WP_000269912.1
Coordinates 37978..38325 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KZW89_RS28570 Protein ID WP_001673379.1
Coordinates 37679..37978 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW89_RS28540 (KZW89_28540) 32862..33110 + 249 WP_000730008.1 hypothetical protein -
KZW89_RS28545 (KZW89_28550) 34237..34428 + 192 WP_000183351.1 hypothetical protein -
KZW89_RS28550 (KZW89_28555) 34425..35567 + 1143 WP_021542821.1 ORF6N domain-containing protein -
KZW89_RS28555 (KZW89_28560) 36377..36649 - 273 WP_000148348.1 helix-turn-helix domain-containing protein -
KZW89_RS28560 (KZW89_28565) 36713..37093 - 381 WP_000061763.1 hypothetical protein -
KZW89_RS28565 (KZW89_28570) 37361..37645 + 285 WP_021542820.1 hypothetical protein -
KZW89_RS28570 (KZW89_28575) 37679..37978 - 300 WP_001673379.1 XRE family transcriptional regulator Antitoxin
KZW89_RS28575 (KZW89_28580) 37978..38325 - 348 WP_000269912.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZW89_RS28580 (KZW89_28585) 38570..38833 - 264 WP_000424604.1 hypothetical protein -
KZW89_RS28585 (KZW89_28590) 38857..39144 - 288 WP_000356589.1 hypothetical protein -
KZW89_RS28590 (KZW89_28595) 39788..40768 - 981 WP_235840137.1 plasmid replication initiator RepA -
KZW89_RS28595 (KZW89_28600) 40950..41504 - 555 WP_001199267.1 recombinase family protein -
KZW89_RS28600 (KZW89_28605) 41586..42539 + 954 WP_021543120.1 hypothetical protein -
KZW89_RS28605 (KZW89_28610) 42542..43075 + 534 WP_000972139.1 tail fiber assembly protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..48077 48077


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13545.51 Da        Isoelectric Point: 8.8337

>T211279 WP_000269912.1 NZ_CP080230:c38325-37978 [Escherichia coli O141:H4]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Download         Length: 348 bp

>T211279 NZ_CP104796:c4858215-4857298 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11069.90 Da        Isoelectric Point: 5.8899

>AT211279 WP_001673379.1 NZ_CP080230:c37978-37679 [Escherichia coli O141:H4]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFHV

Download         Length: 300 bp

>AT211279 NZ_CP104796:c4858979-4858221 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAAGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References