Detailed information of TA system TA211277

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-agrB/-
• Location: 73659..74075
• Replicon: plasmid pP136-5
• Accession: NZ_CP080228
• Organism: Escherichia coli O141:H4 strain P13-6

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KZW89_RS27770
• Protein ID: WP_228932012.1
• Coordinates: 73956..74075 (+)
• Length: 40 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	73659..73868 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW89_RS27740	68883..69146	+	264	WP_000063165.1	hypothetical protein	-
KZW89_RS27745	69318..69740	+	423	WP_001398192.1	hypothetical protein	-
KZW89_RS29075	70661..70828	-	168	WP_001372210.1	hypothetical protein	-
KZW89_RS27755	71018..71491	+	474	WP_001122138.1	type II toxin-antitoxin system PemK/MazF family toxin	-
KZW89_RS27760	71914..72279	+	366	WP_000091308.1	hypothetical protein	-
KZW89_RS27765	72279..73466	+	1188	WP_000937591.1	IS91 family transposase	-
-	73656..73868	+	213	NuclAT_0	-	-
-	73656..73868	+	213	NuclAT_0	-	-
-	73656..73868	+	213	NuclAT_0	-	-
-	73656..73868	+	213	NuclAT_0	-	-
-	73659..73868	-	210	-	-	Antitoxin
KZW89_RS29080	73856..74008	+	153	Protein_85	FlmC family protein	-
KZW89_RS27770	73956..74075	+	120	WP_228932012.1	Hok/Gef family protein	Toxin
KZW89_RS29085	74546..74676	-	131	Protein_87	hypothetical protein	-
KZW89_RS27775	74725..75021	+	297	WP_001272251.1	hypothetical protein	-
KZW89_RS27780	75132..75953	+	822	WP_001234419.1	DUF932 domain-containing protein	-
KZW89_RS27785	76250..76897	-	648	WP_000614935.1	transglycosylase SLT domain-containing protein	-
KZW89_RS27790	77163..77546	+	384	WP_120150413.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KZW89_RS27795	77737..78423	+	687	WP_000332485.1	PAS domain-containing protein	-
KZW89_RS27800	78519..78746	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD	1..86378	86378
	-	flank	IS/Tn	-	-	72279..73466	1187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 40 a.a.
• Molecular weight: 4425.22 Da
• Isoelectric Point: 8.2691

>T211277 WP_228932012.1 NZ_CP080228:73956-74075 [Escherichia coli O141:H4]
VCCTLLIFTLLTRNRLCEIRLKDGYREVTASMAYESGGK

Nucleotide

• Length: 120 bp

>T211277 NZ_CP104796:c4613017-4612484 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 210 bp

>AT211277 NZ_CP080228:c73868-73659 [Escherichia coli O141:H4]
GTGGACTAGACATGCAGAGGCCTCGTGGGTTAATGAAAATTAACTACGGGGCTTTTGTCCTTCTGCCACACGACAGGATA
ACAAACCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGCTGACCACA
TTCACTTTCCCTGAAAATAATCCGGTCGTTCAGCCAGTTCACGGGCAATC

Structures

Antitoxin