Detailed information of TA system TA211267

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 57335..57599
• Replicon: plasmid pP136-2
• Accession: NZ_CP080225
• Organism: Escherichia coli O141:H4 strain P13-6

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: KZW89_RS26085
• Protein ID: WP_001331364.1
• Coordinates: 57447..57599 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	57335..57392 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZW89_RS26070 (52574)	52574..54865	-	2292	WP_001289276.1	F-type conjugative transfer protein TrbC	-
KZW89_RS26075 (54858)	54858..55928	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
KZW89_RS26080 (55947)	55947..57155	-	1209	WP_000121274.1	IncI1-type conjugal transfer protein TrbA	-
- (57335)	57335..57392	-	58	NuclAT_0	-	Antitoxin
- (57335)	57335..57392	-	58	NuclAT_0	-	Antitoxin
- (57335)	57335..57392	-	58	NuclAT_0	-	Antitoxin
- (57335)	57335..57392	-	58	NuclAT_0	-	Antitoxin
KZW89_RS26085 (57447)	57447..57599	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
KZW89_RS26090 (58222)	58222..58518	+	297	WP_001275298.1	DinQ-like type I toxin DqlB	-
KZW89_RS26095 (58583)	58583..58759	-	177	WP_001054904.1	hypothetical protein	-
KZW89_RS26100 (59151)	59151..59360	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
KZW89_RS26105 (59432)	59432..60094	-	663	WP_000653334.1	plasmid IncI1-type surface exclusion protein ExcA	-
KZW89_RS26110 (60159)	60159..62321	-	2163	WP_000698351.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mef(B) / sul3 / ant(3'')-Ia / cmlA1 / aadA2	-	1..103673	103673

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T211267 WP_001331364.1 NZ_CP080225:57447-57599 [Escherichia coli O141:H4]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T211267 NZ_CP104790:c176535-176137 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCTGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Nucleotide

• Length: 58 bp

>AT211267 NZ_CP080225:c57392-57335 [Escherichia coli O141:H4]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin