Detailed information of TA system TA211261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3851956..3852650
• Replicon: chromosome
• Accession: NZ_CP080223
• Organism: Escherichia coli O141:H4 strain P13-6

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: KZW89_RS19315
• Protein ID: WP_001263489.1
• Coordinates: 3851956..3852354 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: KZW89_RS19320
• Protein ID: WP_000554758.1
• Coordinates: 3852357..3852650 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3847544)	3847544..3847624	-	81	NuclAT_12	-	-
- (3847544)	3847544..3847624	-	81	NuclAT_12	-	-
- (3847544)	3847544..3847624	-	81	NuclAT_12	-	-
- (3847544)	3847544..3847624	-	81	NuclAT_12	-	-
KZW89_RS19290 (3848220)	3848220..3848678	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KZW89_RS19295 (3848939)	3848939..3850396	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
KZW89_RS19300 (3850453)	3850453..3850974	-	522	Protein_3788	peptide chain release factor H	-
KZW89_RS19305 (3850970)	3850970..3851176	-	207	Protein_3789	RtcB family protein	-
KZW89_RS19310 (3851494)	3851494..3851946	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
KZW89_RS19315 (3851956)	3851956..3852354	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KZW89_RS19320 (3852357)	3852357..3852650	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KZW89_RS19325 (3852702)	3852702..3853758	-	1057	Protein_3793	DNA polymerase IV	-
KZW89_RS19330 (3853829)	3853829..3854614	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
KZW89_RS19335 (3854586)	3854586..3856298	+	1713	Protein_3795	flagellar biosynthesis protein FlhA	-
KZW89_RS19340 (3856522)	3856522..3857019	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR / clpV/tssH / tssA / tssA / hcp1/tssD1	3851956..3889986	38030

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T211261 WP_001263489.1 NZ_CP080223:c3852354-3851956 [Escherichia coli O141:H4]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T211261 NZ_CP104788:4788429-4788716 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT211261 WP_000554758.1 NZ_CP080223:c3852650-3852357 [Escherichia coli O141:H4]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT211261 NZ_CP104788:4788703-4789005 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3