Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1879221..1879723 Replicon chromosome
Accession NZ_CP080223
Organism Escherichia coli O141:H4 strain P13-6

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag KZW89_RS09040 Protein ID WP_000767829.1
Coordinates 1879469..1879723 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A2S1Y4D6
Locus tag KZW89_RS09035 Protein ID WP_001259254.1
Coordinates 1879221..1879472 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW89_RS09010 (1874399) 1874399..1875466 - 1068 WP_000080035.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
KZW89_RS09015 (1875466) 1875466..1876536 - 1071 WP_000108986.1 histidinol-phosphate transaminase -
KZW89_RS09020 (1876533) 1876533..1877837 - 1305 WP_120150211.1 histidinol dehydrogenase -
KZW89_RS09025 (1877843) 1877843..1878742 - 900 WP_000131770.1 ATP phosphoribosyltransferase -
KZW89_RS09030 (1878888) 1878888..1878938 - 51 WP_001364200.1 his operon leader peptide -
KZW89_RS09035 (1879221) 1879221..1879472 + 252 WP_001259254.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
KZW89_RS09040 (1879469) 1879469..1879723 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
KZW89_RS09045 (1879806) 1879806..1880630 + 825 WP_000754737.1 SDR family oxidoreductase -
KZW89_RS09050 (1880676) 1880676..1881605 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
KZW89_RS09055 (1881820) 1881820..1881882 + 63 WP_010723108.1 membrane protein YoeI -
KZW89_RS09060 (1881872) 1881872..1883230 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
KZW89_RS09065 (1883408) 1883408..1884466 + 1059 WP_000492313.1 thiosulfate utilization transporter TsuA/YeeE -
KZW89_RS09070 (1884480) 1884480..1884707 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T211248 WP_000767829.1 NZ_CP080223:1879469-1879723 [Escherichia coli O141:H4]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T211248 NZ_CP104788:c1723389-1722067 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9292.57 Da        Isoelectric Point: 4.5822

>AT211248 WP_001259254.1 NZ_CP080223:1879221-1879472 [Escherichia coli O141:H4]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGIGTEKD
IIE

Download         Length: 252 bp

>AT211248 NZ_CP104788:c1723655-1723389 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A2S1Y4D6

References