Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 699906..700599 Replicon chromosome
Accession NZ_CP080223
Organism Escherichia coli O141:H4 strain P13-6

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag KZW89_RS03395 Protein ID WP_000415584.1
Coordinates 699906..700202 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag KZW89_RS03400 Protein ID WP_000650107.1
Coordinates 700204..700599 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW89_RS03360 (694994) 694994..695308 - 315 WP_000958598.1 putative quinol monooxygenase -
KZW89_RS03365 (695339) 695339..695920 - 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
KZW89_RS03370 (696239) 696239..696571 + 333 WP_000917684.1 DUF2645 family protein -
KZW89_RS03375 (696617) 696617..697966 - 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
KZW89_RS03380 (697963) 697963..698622 - 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB -
KZW89_RS03385 (698774) 698774..699166 + 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
KZW89_RS03390 (699219) 699219..699701 + 483 WP_000183505.1 GyrI-like domain-containing protein -
KZW89_RS03395 (699906) 699906..700202 + 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
KZW89_RS03400 (700204) 700204..700599 + 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
KZW89_RS03405 (700732) 700732..702339 + 1608 WP_001295629.1 ABC transporter substrate-binding protein -
KZW89_RS03410 (702477) 702477..704735 + 2259 WP_001281881.1 DNA topoisomerase IV subunit A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - rfaE 639158..704735 65577


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T211245 WP_000415584.1 NZ_CP080223:699906-700202 [Escherichia coli O141:H4]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T211245 NZ_CP104788:c1415668-1415561 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT211245 WP_000650107.1 NZ_CP080223:700204-700599 [Escherichia coli O141:H4]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT211245 NZ_CP104788:1415715-1415781 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References