Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 625576..626303 Replicon chromosome
Accession NZ_CP080223
Organism Escherichia coli O141:H4 strain P13-6

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KZW89_RS03040 Protein ID WP_000550189.1
Coordinates 625576..625890 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KZW89_RS03045 Protein ID WP_000560266.1
Coordinates 625887..626303 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW89_RS03020 (621734) 621734..622720 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
KZW89_RS03025 (622799) 622799..623491 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KZW89_RS03030 (623568) 623568..624071 - 504 WP_001333820.1 M48 family metallopeptidase -
KZW89_RS03035 (624156) 624156..625292 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KZW89_RS03040 (625576) 625576..625890 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KZW89_RS03045 (625887) 625887..626303 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KZW89_RS03050 (626348) 626348..628366 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KZW89_RS03055 (628792) 628792..631143 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T211244 WP_000550189.1 NZ_CP080223:625576-625890 [Escherichia coli O141:H4]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T211244 NZ_CP104788:c1415133-1415026 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT211244 WP_000560266.1 NZ_CP080223:625887-626303 [Escherichia coli O141:H4]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT211244 NZ_CP104788:1415181-1415247 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References