Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 582343..583142 Replicon chromosome
Accession NZ_CP080223
Organism Escherichia coli O141:H4 strain P13-6

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KZW89_RS02815 Protein ID WP_000347273.1
Coordinates 582343..582807 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KZW89_RS02820 Protein ID WP_001307405.1
Coordinates 582807..583142 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZW89_RS02785 (577344) 577344..577778 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KZW89_RS02790 (577796) 577796..578674 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KZW89_RS02795 (578664) 578664..579443 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZW89_RS02800 (579454) 579454..579927 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZW89_RS02805 (579950) 579950..581230 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZW89_RS02810 (581479) 581479..582288 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KZW89_RS02815 (582343) 582343..582807 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZW89_RS02820 (582807) 582807..583142 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZW89_RS02825 (583291) 583291..584862 - 1572 WP_001273753.1 galactarate dehydratase -
KZW89_RS02830 (585237) 585237..586571 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KZW89_RS02835 (586587) 586587..587357 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T211243 WP_000347273.1 NZ_CP080223:c582807-582343 [Escherichia coli O141:H4]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T211243 NZ_CP104788:c1298849-1298562 [Escherichia coli]
ATGTTACCCATTTTGTGGCTACCATCTGCTCGCGATGATTTGCGTCAGATCGTAGCCTATATTGCTAAGGAAAATATTCC
TGCAGCACGCAGACTAAAAATACGGATTGAAACGTCTGTTTTAGCCCTCTCTGAGCATCCATATCTATATCCGCCAAGTG
ATCGAGTATCCGGTTTGCGGGAAATTGTGGTTCACCCTAATTATATCGTTTTGTACCGAGTAGCAGCTTCAAGCATTGAA
ATTGCAAATATTGTGCATGCCCGCCGACAATTTCCCTTCCCTATCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT211243 WP_001307405.1 NZ_CP080223:c583142-582807 [Escherichia coli O141:H4]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT211243 NZ_CP104788:c1299040-1298849 [Escherichia coli]
ATGAGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTACGCGC
TAAAGTGGCGTCAAGCCTTGCTGACCCTCGTCCCTCAATTCCACATGATGAGGTAATGGCTGAAATGGAGAATCTTATTG
CTCAAATTGCTGTAACTAACAGGAGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References