Detailed information of TA system TA211224

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 97776..98202
• Replicon: plasmid pPK8566-tetX
• Accession: NZ_CP080175
• Organism: Escherichia coli strain PK8566

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KZY09_RS25630
• Protein ID: WP_001372321.1
• Coordinates: 97776..97901 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	97978..98202 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY09_RS25615 (93282)	93282..94103	-	822	WP_001234469.1	DUF932 domain-containing protein	-
KZY09_RS25620 (94224)	94224..94511	-	288	WP_000107544.1	hypothetical protein	-
KZY09_RS26110 (94536)	94536..94742	-	207	WP_000547968.1	hypothetical protein	-
KZY09_RS26115 (94812)	94812..94985	+	174	Protein_108	hypothetical protein	-
KZY09_RS25625 (95714)	95714..97381	+	1668	WP_001762494.1	group II intron reverse transcriptase/maturase	-
KZY09_RS25630 (97776)	97776..97901	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KZY09_RS26120 (97843)	97843..97992	-	150	Protein_111	plasmid maintenance protein Mok	-
- (97980)	97980..98045	+	66	NuclAT_0	-	-
- (97980)	97980..98045	+	66	NuclAT_0	-	-
- (97980)	97980..98045	-	66	NuclAT_1	-	-
- (97978)	97978..98202	+	225	NuclAT_0	-	-
- (97978)	97978..98202	-	225	NuclAT_0	-	Antitoxin
- (97978)	97978..98202	-	225	NuclAT_0	-	Antitoxin
- (97978)	97978..98202	-	225	NuclAT_0	-	Antitoxin
- (97978)	97978..98202	-	225	NuclAT_0	-	Antitoxin
KZY09_RS25635 (98014)	98014..98202	+	189	WP_001299721.1	hypothetical protein	-
KZY09_RS25640 (98171)	98171..98933	-	763	Protein_113	plasmid SOS inhibition protein A	-
KZY09_RS25645 (98930)	98930..99364	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KZY09_RS25650 (99419)	99419..101377	-	1959	WP_001825193.1	ParB/RepB/Spo0J family partition protein	-
KZY09_RS25655 (101443)	101443..101676	-	234	WP_000005990.1	DUF905 family protein	-
KZY09_RS25660 (101739)	101739..102278	-	540	WP_001825195.1	single-stranded DNA-binding protein	-
KZY09_RS25665 (102304)	102304..102510	-	207	WP_000275858.1	hypothetical protein	-
KZY09_RS26125 (102580)	102580..102753	+	174	Protein_119	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T211224 WP_001372321.1 NZ_CP080175:c97901-97776 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T211224 NZ_CP104766:324287-324655 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT211224 NZ_CP080175:c98202-97978 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin