Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | hok-sok/- |
| Location | 69083..69347 | Replicon | plasmid pPK8566-117kb |
| Accession | NZ_CP080174 | ||
| Organism | Escherichia coli strain PK8566 | ||
Toxin (Protein)
| Gene name | pndA | Uniprot ID | E6BRV3 |
| Locus tag | KZY09_RS24850 | Protein ID | WP_001303307.1 |
| Coordinates | 69195..69347 (+) | Length | 51 a.a. |
Antitoxin (RNA)
| Gene name | pndB | ||
| Locus tag | - | ||
| Coordinates | 69083..69145 (-) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KZY09_RS24835 (64322) | 64322..66613 | - | 2292 | WP_001289271.1 | F-type conjugative transfer protein TrbC | - |
| KZY09_RS24840 (66606) | 66606..67676 | - | 1071 | WP_000151575.1 | IncI1-type conjugal transfer protein TrbB | - |
| KZY09_RS24845 (67695) | 67695..68903 | - | 1209 | WP_000121273.1 | IncI1-type conjugal transfer protein TrbA | - |
| - (69083) | 69083..69145 | - | 63 | NuclAT_0 | - | Antitoxin |
| - (69083) | 69083..69145 | - | 63 | NuclAT_0 | - | Antitoxin |
| - (69083) | 69083..69145 | - | 63 | NuclAT_0 | - | Antitoxin |
| - (69083) | 69083..69145 | - | 63 | NuclAT_0 | - | Antitoxin |
| KZY09_RS24850 (69195) | 69195..69347 | + | 153 | WP_001303307.1 | Hok/Gef family protein | Toxin |
| KZY09_RS24855 (69419) | 69419..69670 | - | 252 | WP_001291965.1 | hypothetical protein | - |
| - (70057) | 70057..70108 | - | 52 | NuclAT_1 | - | - |
| - (70057) | 70057..70108 | - | 52 | NuclAT_1 | - | - |
| - (70057) | 70057..70108 | - | 52 | NuclAT_1 | - | - |
| - (70057) | 70057..70108 | - | 52 | NuclAT_1 | - | - |
| KZY09_RS24860 (70824) | 70824..72052 | + | 1229 | WP_088895425.1 | IS3-like element IS2 family transposase | - |
| KZY09_RS24865 (72498) | 72498..72707 | + | 210 | WP_000062603.1 | HEAT repeat domain-containing protein | - |
| KZY09_RS24870 (72779) | 72779..73441 | - | 663 | WP_000644794.1 | plasmid IncI1-type surface exclusion protein ExcA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..117749 | 117749 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 51 a.a. Molecular weight: 5817.21 Da Isoelectric Point: 8.7948
>T211220 WP_001303307.1 NZ_CP080174:69195-69347 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR
Download Length: 153 bp
>T211220 NZ_CP104762:1108791-1109069 [Enterococcus raffinosus]
ATGCTGACGATTGAGTACACTTCTATATTCAAAAGAGATTATAAAAGAATGGTGAAAAAACACTACAATATGAAAAAGTT
AGAAAAAGTAGTTAAGTTGCTTGTCACAAACAACCAAGATGAATTGATAAGAAAATATAAAGACCATGCTTTAAAAGGGA
ACTGGAAAGGTTACAGAGAACTACATTTAGATAACGACTGGTTACTAATTTACAAGATTGACGACAAAAATTTAATCTTA
ACAATGACACGTACAGGAAGTCATGATGAACTTTTGTGA
ATGCTGACGATTGAGTACACTTCTATATTCAAAAGAGATTATAAAAGAATGGTGAAAAAACACTACAATATGAAAAAGTT
AGAAAAAGTAGTTAAGTTGCTTGTCACAAACAACCAAGATGAATTGATAAGAAAATATAAAGACCATGCTTTAAAAGGGA
ACTGGAAAGGTTACAGAGAACTACATTTAGATAACGACTGGTTACTAATTTACAAGATTGACGACAAAAATTTAATCTTA
ACAATGACACGTACAGGAAGTCATGATGAACTTTTGTGA
Antitoxin
Download Length: 63 bp
>AT211220 NZ_CP080174:c69145-69083 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
