Detailed information of TA system TA211212

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 119084..119505
• Replicon: plasmid pPK8566-169kb
• Accession: NZ_CP080173
• Organism: Escherichia coli strain PK8566

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KZY09_RS24185
• Protein ID: WP_096937776.1
• Coordinates: 119084..119209 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	119307..119505 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY09_RS24150 (114149)	114149..114364	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
KZY09_RS24155 (114500)	114500..115147	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
KZY09_RS24160 (115339)	115339..115722	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KZY09_RS24165 (116074)	116074..116664	+	591	WP_166494936.1	transglycosylase SLT domain-containing protein	-
KZY09_RS24170 (116959)	116959..117780	-	822	WP_001234475.1	DUF932 domain-containing protein	-
KZY09_RS24175 (117899)	117899..118186	-	288	WP_000107546.1	hypothetical protein	-
KZY09_RS24180 (118487)	118487..118783	+	297	Protein_124	hypothetical protein	-
KZY09_RS24185 (119084)	119084..119209	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
KZY09_RS26060 (119151)	119151..119300	-	150	Protein_126	DUF5431 family protein	-
- (119307)	119307..119505	-	199	NuclAT_0	-	Antitoxin
- (119307)	119307..119505	-	199	NuclAT_0	-	Antitoxin
- (119307)	119307..119505	-	199	NuclAT_0	-	Antitoxin
- (119307)	119307..119505	-	199	NuclAT_0	-	Antitoxin
KZY09_RS24190 (119474)	119474..120236	-	763	Protein_127	plasmid SOS inhibition protein A	-
KZY09_RS24195 (120233)	120233..120667	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
KZY09_RS24200 (120722)	120722..122680	-	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
KZY09_RS24205 (122746)	122746..122979	-	234	WP_000005990.1	DUF905 family protein	-
KZY09_RS24210 (123036)	123036..123575	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
KZY09_RS24215 (123893)	123893..124402	+	510	WP_071600123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / tet(A) / sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..169561	169561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T211212 WP_096937776.1 NZ_CP080173:c119209-119084 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T211212 NZ_CP104757:c3046198-3045878 [Pectobacterium aroidearum]
ATGCAAACCATACCGGAAATCCCGTCACGGGAGGAGCAATCCTGCCCGTCACCTATAGCCGTCTGGCAGCAACTGCTTAC
CTACCTGTTAGAAAAGCACTACGGCCTGACGCTGAACGACACACCGTTCTGTGAGGAAAACGTCATTCAGGCGCATATCG
ATGCTGGCGTCACGCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAATACGAACTGGTGCGTATCGATCGCGACGGTTTT
AACTGGCAGGAGCAATCACCGTTTCTCACCGCCGTTGATATTCTCCGTGCCAGACGCGCTACCGGCTTGCTTAAAGCATA
A

Antitoxin

Nucleotide

• Length: 199 bp

>AT211212 NZ_CP080173:c119505-119307 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin