Detailed information of TA system TA211208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4827879..4828481
• Replicon: chromosome
• Accession: NZ_CP080172
• Organism: Escherichia coli strain PK8566

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KZY09_RS23405
• Protein ID: WP_000897305.1
• Coordinates: 4827879..4828190 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZY09_RS23410
• Protein ID: WP_000356397.1
• Coordinates: 4828191..4828481 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY09_RS23375 (4822909)	4822909..4823694	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
KZY09_RS23380 (4823793)	4823793..4824392	+	600	WP_001295269.1	glucose-1-phosphatase	-
KZY09_RS23385 (4824386)	4824386..4825258	+	873	WP_000920762.1	virulence factor BrkB family protein	-
KZY09_RS23390 (4825255)	4825255..4825692	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KZY09_RS23395 (4825737)	4825737..4826678	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KZY09_RS23400 (4826742)	4826742..4827650	-	909	WP_001162704.1	alpha/beta hydrolase	-
KZY09_RS23405 (4827879)	4827879..4828190	+	312	WP_000897305.1	hypothetical protein	Toxin
KZY09_RS23410 (4828191)	4828191..4828481	+	291	WP_000356397.1	NadS family protein	Antitoxin
KZY09_RS23415 (4829086)	4829086..4829304	+	219	WP_001315930.1	CopG family transcriptional regulator	-
KZY09_RS23420 (4829524)	4829524..4829766	+	243	WP_001087409.1	protein YiiF	-
KZY09_RS23425 (4830096)	4830096..4831025	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KZY09_RS23430 (4831022)	4831022..4831657	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KZY09_RS23435 (4831654)	4831654..4832555	-	902	Protein_4601	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T211208 WP_000897305.1 NZ_CP080172:4827879-4828190 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T211208 NZ_CP104757:747852-748226 [Pectobacterium aroidearum]
ATGCAACTGCTTTCATTGATTGTGCATGGCCTATTAGTATTGCTCATTCTTCTGGCGCCGTGGCCGGACGGCTATGCATG
GCTGTGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTATCCGTAGCCAAAGGAATATCAAATCCCGGCAGGGGGAAA
TCGTTTTGCTCAGCGAAACGACCCTGAACTGGCGGCAGCAGGAATGGCAGATCGTTAAACGACCGTGGTTGCTGAAAAAT
GGCGTATTGCTGTCATTGCAGGCAGTGAACGGTAAAGACAGACAACAGCTGTGGCTGGCATCGGACAGCATGGGCGATGA
CGAATGGCGTCATCTGCGTCAGCTTCTTTTGCAGCAGAAAAACTGGGCGAGATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT211208 WP_000356397.1 NZ_CP080172:4828191-4828481 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT211208 NZ_CP104757:747566-747832 [Pectobacterium aroidearum]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGGGGTATGCGTGAGTTGGATATCGCGATCATGCCGTT
TTTTGAGCATGACTACGACACACTCAGTGACAGCGATAAGCGCACCTTTGTGCGCCTGTTACAAAGCGATGATCCCGATT
TATTCAACTGGCTGATGAACCACGGCGAGCCGAAAGACGGAGAGCTGAAACGCATGATTTCTCTTATTCAAACGCGAAAT
AAAGATCGTGGCCCAGTGGCAATGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7