Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3989786..3990585 Replicon chromosome
Accession NZ_CP080172
Organism Escherichia coli strain PK8566

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag KZY09_RS19325 Protein ID WP_000347266.1
Coordinates 3990121..3990585 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KZY09_RS19320 Protein ID WP_001307405.1
Coordinates 3989786..3990121 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY09_RS19305 (3985571) 3985571..3986341 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
KZY09_RS19310 (3986357) 3986357..3987691 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KZY09_RS19315 (3988066) 3988066..3989637 + 1572 WP_001273741.1 galactarate dehydratase -
KZY09_RS19320 (3989786) 3989786..3990121 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZY09_RS19325 (3990121) 3990121..3990585 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZY09_RS19330 (3990640) 3990640..3991449 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KZY09_RS19335 (3991698) 3991698..3992978 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZY09_RS19340 (3993001) 3993001..3993474 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZY09_RS19345 (3993485) 3993485..3994264 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZY09_RS19350 (3994254) 3994254..3995132 + 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
KZY09_RS19355 (3995150) 3995150..3995584 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3977519..3990585 13066


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T211205 WP_000347266.1 NZ_CP080172:3990121-3990585 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T211205 NZ_CP104757:353550-353876 [Pectobacterium aroidearum]
ATGACCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAATTAGGGCATCCTGTCAGAGAACAATTCAA
AAAAAAGCTAGTTGAGCGTTTAGAAAATCCTCGTGTTCCTTCAGCCCAGCTCCATGGTCGTAAAGACCAGTATAAAATTA
AACTGAAGTCCGCTGGTTACAGGCTGGTGTATTTGGTTCAGGATGAGACCATTACGGTGATGGTCATGGGGGTTGGAAAG
CGCGAAGGTAGTCAGGTTTATTCTGATACTAAATCTGGTCTTTTGCGAGTTATCTATTCCTCGTCGGCAGTTTTTCTTGC
CGTGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT211205 WP_001307405.1 NZ_CP080172:3989786-3990121 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT211205 NZ_CP104757:353306-353560 [Pectobacterium aroidearum]
ATGGCTTTCAATATCCTAACTAACACAGCGGCCAGTATCACTGAACTGAAACGCGACCCAATGGGGACAGTACGGGCTGC
TGAAGGTGAGACTATCGCTATACTGAACCGGAATGAGCCTGCGTTCTATTGTGTGCCTCCTGCCATGTTCGCTTATTTAA
TGGAATTGGCTGAAGATGCAGAGTTGGGGCGTACTGTTGATGATCGTATGTCAGAGTTAGCTGACGCGGAAGAGATTTCG
CTCGATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References