Detailed information of TA system TA211199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2662216..2662718
• Replicon: chromosome
• Accession: NZ_CP080172
• Organism: Escherichia coli strain PK8566

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KZY09_RS13130
• Protein ID: WP_105078014.1
• Coordinates: 2662216..2662470 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: KZY09_RS13135
• Protein ID: WP_001259255.1
• Coordinates: 2662467..2662718 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY09_RS13100 (2657231)	2657231..2657458	-	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
KZY09_RS13105 (2657472)	2657472..2658530	-	1059	WP_000492346.1	thiosulfate utilization transporter TsuA/YeeE	-
KZY09_RS13110 (2658709)	2658709..2660067	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
KZY09_RS13115 (2660057)	2660057..2660119	-	63	WP_010723108.1	membrane protein YoeI	-
KZY09_RS13120 (2660334)	2660334..2661284	-	951	WP_105078013.1	LysR substrate-binding domain-containing protein	-
KZY09_RS13125 (2661309)	2661309..2662133	-	825	WP_000754737.1	SDR family oxidoreductase	-
KZY09_RS13130 (2662216)	2662216..2662470	-	255	WP_105078014.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KZY09_RS13135 (2662467)	2662467..2662718	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KZY09_RS13140 (2663001)	2663001..2663051	+	51	WP_001364200.1	his operon leader peptide	-
KZY09_RS13145 (2663197)	2663197..2664096	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KZY09_RS13150 (2664102)	2664102..2665406	+	1305	WP_021526186.1	histidinol dehydrogenase	-
KZY09_RS13155 (2665403)	2665403..2666473	+	1071	WP_000108937.1	histidinol-phosphate transaminase	-
KZY09_RS13160 (2666473)	2666473..2667540	+	1068	WP_029397756.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10130.53 Da
• Isoelectric Point: 7.2415

>T211199 WP_105078014.1 NZ_CP080172:c2662470-2662216 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHCLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T211199 NZ_CP104755:4172099-4172398 [Shewanella putrefaciens]
ATGAATAATAAACGCTATAAACTGAGTAGGTTATCTCAGACTCACCTACAACAGATTAAAGACTACACCCTTCAACACTT
TTCTGAGTCGCAGTGGCACAAATATAAAGAAAGCCTTATCTCCGGTTTGCAAATGTTAGCCGATAACCCCGGACTAGGTA
GAAGCTGCGAAGATATTTACCCCAACGGATTCTACTTTCCGATTGCTAAACACACCGCCTACTTCACAAAAGAAGACGAC
TTCATCCTCATCGTTGCCCTGTTAGGCCAGCCACAACTACCACAAAACCACTTGAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT211199 WP_001259255.1 NZ_CP080172:c2662718-2662467 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT211199 NZ_CP104755:4171848-4172090 [Shewanella putrefaciens]
GTGCAAACATTAACCGCCAATGAAGCCAAACGAAATTTTGGTGAATTGCTACTAAACGCTCAACGTGAACCCATCAAAAT
CAGCAAAAACAGTAAAGATGCGGTTGTGGTTATGTCTATCCACGATTACGAACAGCTTGAGGCGATGAAAGCCGACTATC
TGAAGCACTGTTTCAACGCAGCCAAAGAAGATTTAGCGAAGGGCAATGTGGTTGATGGTGAAGATTTTCTTAACGCCCTG
TAG

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0