Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 847350..847891 Replicon chromosome
Accession NZ_CP080172
Organism Escherichia coli strain PK8566

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KZY09_RS04080 Protein ID WP_000615976.1
Coordinates 847350..847628 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KZY09_RS04085 Protein ID WP_000729704.1
Coordinates 847631..847891 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY09_RS04065 (844932) 844932..845510 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
KZY09_RS04070 (845716) 845716..846483 + 768 WP_000333380.1 class II glutamine amidotransferase -
KZY09_RS04075 (846454) 846454..847194 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KZY09_RS04080 (847350) 847350..847628 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KZY09_RS04085 (847631) 847631..847891 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KZY09_RS04090 (848077) 848077..848850 + 774 WP_000543895.1 C40 family peptidase -
KZY09_RS04095 (848907) 848907..849263 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
KZY09_RS04100 (849256) 849256..849534 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KZY09_RS04105 (849639) 849639..851351 - 1713 Protein_801 flagellar biosynthesis protein FlhA -
KZY09_RS04110 (851323) 851323..852108 + 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - hcp1/tssD1 / tssM / vgrG/tssI / rhs/PAAR / gmhA/lpcA 822095..849534 27439


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T211180 WP_000615976.1 NZ_CP080172:c847628-847350 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T211180 NZ_CP080172:c847628-847350 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT211180 WP_000729704.1 NZ_CP080172:c847891-847631 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT211180 NZ_CP080172:c847891-847631 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References