Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 290924..291421 Replicon chromosome
Accession NZ_CP080172
Organism Escherichia coli strain PK8566

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KZY09_RS01370 Protein ID WP_001173343.1
Coordinates 291248..291421 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KZY09_RS01365 Protein ID WP_000398619.1
Coordinates 290924..291220 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY09_RS01345 (287655) 287655..288374 - 720 WP_064525723.1 two-component system response regulator DcuR -
KZY09_RS01350 (288371) 288371..290002 - 1632 WP_001216461.1 sensor histidine kinase -
KZY09_RS01355 (290183) 290183..290413 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
KZY09_RS01360 (290425) 290425..290697 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
KZY09_RS01365 (290924) 290924..291220 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KZY09_RS01370 (291248) 291248..291421 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KZY09_RS01375 (291540) 291540..293057 - 1518 WP_001295074.1 lysine--tRNA ligase -
KZY09_RS01380 (293294) 293294..294751 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 290924..300275 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T211178 WP_001173343.1 NZ_CP080172:291248-291421 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T211178 NZ_CP104733:1570347-1570661 [Pectobacterium sp. F1-1]
ATGCAATTCACTGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATCGG
GCAATTAAATCGTCGGATAGTGATCCCATTGCTCCCTATTGAAAAGTATCCGGCAGGCCGCCGACCGGATCGCCTTGTCC
CCGTCATCAGGCTGACGGATGGCAAAGAATACGCCGTAATGACCCACGAACTGGCAAGTATCCCTGTTCAGGCTCTGGGG
ACGGTGTTTTGTGATGTGTCGCAGTACCGCACTCAGGTAAAGGCGGCAATAGACTTCCTCATCGACGGGTTCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT211178 WP_000398619.1 NZ_CP080172:290924-291220 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT211178 NZ_CP104733:1570108-1570344 [Pectobacterium sp. F1-1]
ATGACCGCGAAACAACGCAACACGCAGAGCGTGACCATGACAGTAGAGCGTGCCTTATTGGTAAGAGCGCGTGAAGCGGG
TATTAATCTGAGTGCTACCCTGACAACCGCGCTGGATGCAGAGCTTCGCTCTCATGAAGCGAAAAAATGGCAGGAAGAAA
ACAGGGAGGCTCTCGCAGCACTAAATCGTTTTCATGATGAGCAGGGTTGTTTCAGCGATGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References