Detailed information of TA system TA211168

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 128489..128910
• Replicon: plasmid pPK8275-180kb
• Accession: NZ_CP080165
• Organism: Escherichia coli strain PK8275

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KZY08_RS26340
• Protein ID: WP_096937776.1
• Coordinates: 128489..128614 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	128712..128910 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY08_RS26305 (123554)	123554..123769	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
KZY08_RS26310 (123905)	123905..124552	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
KZY08_RS26315 (124744)	124744..125127	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KZY08_RS26320 (125467)	125467..126069	+	603	WP_077248356.1	transglycosylase SLT domain-containing protein	-
KZY08_RS26325 (126364)	126364..127185	-	822	WP_001234475.1	DUF932 domain-containing protein	-
KZY08_RS26330 (127304)	127304..127591	-	288	WP_000107546.1	hypothetical protein	-
KZY08_RS26335 (127892)	127892..128188	+	297	Protein_139	hypothetical protein	-
KZY08_RS26340 (128489)	128489..128614	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
KZY08_RS28400 (128556)	128556..128705	-	150	Protein_141	DUF5431 family protein	-
- (128712)	128712..128910	-	199	NuclAT_0	-	Antitoxin
- (128712)	128712..128910	-	199	NuclAT_0	-	Antitoxin
- (128712)	128712..128910	-	199	NuclAT_0	-	Antitoxin
- (128712)	128712..128910	-	199	NuclAT_0	-	Antitoxin
KZY08_RS26345 (128879)	128879..129641	-	763	Protein_142	plasmid SOS inhibition protein A	-
KZY08_RS26350 (129638)	129638..130072	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
KZY08_RS26355 (130127)	130127..132085	-	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
KZY08_RS26360 (132151)	132151..132384	-	234	WP_000005990.1	DUF905 family protein	-
KZY08_RS26365 (132441)	132441..132980	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
KZY08_RS26370 (133298)	133298..133807	+	510	WP_071600123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / dfrA14 / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..180290	180290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T211168 WP_096937776.1 NZ_CP080165:c128614-128489 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T211168 NZ_CP104729:c88326-87781 [Lysinibacillus fusiformis]
ATGAAAAAGACAGCTATTTTAGTAGATGAAATGAACGTGATTCAACAACTCCGACGTTTAGGAATCCATGGATTCAATCC
CTGGAATGCATTTTATCAGGCGTTACAACGTTGGAATGGTGATAAGAAAGGTGTTAAAGCATTTTATTGTGCTAACGTAC
CTAAAGAAGATGCAGAACATGAAAAGCGTAATAATTTCTTTGAAGGATTAATAAATCATAACATCAAAGTATCAGTAGGT
ACTGTTGTATATGACTCAAACTCAAGACGGATAGCAAAAGGTGTAGATGTTGCCTTAGCGCTGGACATCGTAGAACATGC
TAGAAGCGGTGTACAAGATATTTTCATCTGTACAGGTGATGCTGATGTTGTGCCTGCCATTATTCGCGCACAGGAGCTCG
GTAGTCGTGTTCACGTTGTAGTTTCCAGGGCTATCCCAGCTAAAAATATCACCCTTGCAGCTGATTCAGTGGTTAGATTA
GAGGATATCTTAGAAACCATGCCTCCTCACCGCATTAAATACTTTGCTAAGGAGCGTGTGAGCTAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT211168 NZ_CP080165:c128910-128712 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin