Detailed information of TA system TA211165

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90335..90589
• Replicon: plasmid pPK8275-180kb
• Accession: NZ_CP080165
• Organism: Escherichia coli strain PK8275

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KZY08_RS26115
• Protein ID: WP_001312851.1
• Coordinates: 90335..90484 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	90528..90589 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY08_RS26090 (85617)	85617..88622	+	3006	WP_001143760.1	Tn3-like element Tn3 family transposase	-
KZY08_RS26095 (88555)	88555..89020	-	466	Protein_90	plasmid replication initiator RepA	-
KZY08_RS26100 (89013)	89013..89495	-	483	WP_001273588.1	hypothetical protein	-
KZY08_RS28390 (89488)	89488..89535	-	48	WP_229471593.1	hypothetical protein	-
KZY08_RS28395 (89526)	89526..89777	+	252	WP_223195197.1	replication protein RepA	-
KZY08_RS26110 (89794)	89794..90051	-	258	WP_000083845.1	replication regulatory protein RepA	-
KZY08_RS26115 (90335)	90335..90484	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (90528)	90528..90589	+	62	NuclAT_1	-	Antitoxin
- (90528)	90528..90589	+	62	NuclAT_1	-	Antitoxin
- (90528)	90528..90589	+	62	NuclAT_1	-	Antitoxin
- (90528)	90528..90589	+	62	NuclAT_1	-	Antitoxin
KZY08_RS26120 (90845)	90845..90919	-	75	Protein_96	endonuclease	-
KZY08_RS26125 (91165)	91165..91377	-	213	WP_001299730.1	ANR family transcriptional regulator	-
KZY08_RS26130 (91513)	91513..92073	-	561	WP_000139315.1	fertility inhibition protein FinO	-
KZY08_RS26135 (92176)	92176..93036	-	861	WP_000704513.1	alpha/beta hydrolase	-
KZY08_RS26140 (93095)	93095..93841	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / dfrA14 / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..180290	180290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T211165 WP_001312851.1 NZ_CP080165:c90484-90335 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T211165 NZ_CP104727:6661844-6662680 [Pseudomonas citronellolis]
ATGAGCACGGCGGCCGCCTGGCAGGCCGACCTGGAACGCGACTTCCCGCAGCGCCTGGTGCGCCGCGGCGAGGTCGTCAG
CGCCTGGCGCGAGGCCGGCGCGGCCGATGCCCCGGCGCTGGTGCTGCTGCACGGCATCGGCTCCGGCGCCGCCTCCTGGC
TGCCGCTGGCGCAGTGCCTGAAGGCGCGCGCGCGGCTCATCGCCTGGGACGCCCCCGGCTACGGCGAATCCACGCCGCTG
GCCGAGGCCGCGCCGCGGGCGGAAGCCTACATGCAGCGCCTGCGCGAGCTGCTGGACGCGCTGGACGTGCAGCGCTGCGT
GCTGCTCGGCCATTCCCTGGGAGCGCTGACGGCCCTCGCCCTGGCCAACGCCGAGCCGCGACTCATTGCCCACCTGCTGC
TGCTGAGCCCGGCGCGCGGCTACGGCGCACGCCCGGACGAAGGCGAGGCGGTGCGCAGCCGGCGCCTGGCCGCGCTGGCC
AGCGAGGGCATCGCCGGCCTCGCCGAACGCCGCGCCCCGGCCATGCTCGGCGCCAGCCCGAGTGCCGAGGCGCTGGCCTG
GGTGCGCGGGAACATGGCGCGGCTCAAGCCCCACGGCTACCGCCAGGCGGTGGAGCTGCTGTGCGGCGACGACCTGCTGC
GCCTGGGCCGCCCGCCCATACCCGTCGAGGTGCTGTGCGGCAGCGAGGACGGCATCACCCCGCCGGCCGCCTGCCGCGAA
GTCGCCGCGGCGCTGGCGGCACCGTTCCGGCTAGTCCCCGGCGCCGGCCACGCCAGCCCCATCGAATGCCCCGAAGCCCT
GGCCGGACACCTCGTCCGCTTCCTCGACAAGGACTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT211165 NZ_CP080165:90528-90589 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin