Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3233989..3234551 Replicon chromosome
Accession NZ_CP080163
Organism Escherichia coli strain PK8275

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag KZY08_RS15490 Protein ID WP_000605676.1
Coordinates 3233989..3234267 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag KZY08_RS15495 Protein ID WP_000781370.1
Coordinates 3234267..3234551 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY08_RS15465 (3229575) 3229575..3230006 - 432 WP_000152305.1 peroxiredoxin OsmC -
KZY08_RS15470 (3230351) 3230351..3230566 + 216 WP_000495766.1 biofilm-dependent modulation protein -
KZY08_RS15475 (3230668) 3230668..3230805 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
KZY08_RS15480 (3230962) 3230962..3232659 + 1698 WP_000433464.1 malate dehydrogenase -
KZY08_RS15485 (3232793) 3232793..3233803 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
KZY08_RS15490 (3233989) 3233989..3234267 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZY08_RS15495 (3234267) 3234267..3234551 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
KZY08_RS15500 (3234779) 3234779..3235432 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
KZY08_RS15505 (3235425) 3235425..3236309 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
KZY08_RS15510 (3236322) 3236322..3239369 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T211155 WP_000605676.1 NZ_CP080163:3233989-3234267 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T211155 NZ_CP104727:c1001784-1001374 [Pseudomonas citronellolis]
GTGAAATACCTGCTCGACACCAACATCCTCATCTACCTGCTGAAGAACCGCCCGGAATCCGTGGCCCGACGGGTCAACGC
CCTGCCCGCCGACGCCGGCCTGTGCATGTCCTTCTTCACCTACGCAGAACTGCTCAAGGGCGCCGAACGCAGCACCCGGC
GCAGCGAGGTCCTGCGCCAGCTGGAGCGCCTGACGCGCCAGGTGCCGGTGGTCTACGACGGTACCCCACGCCTGTGCGAG
CACTACGCGACGCAGTTCACCCGCCTCAAGCTGGCCGGCACGCCGATCGGCGCCAACGACCTATGGATCGCCTGCCACGC
CCTGGCGCTGGAGGCGACCCTGGTGACGCACAACACCCGCGAGTTCGAACGCATCGACGGGCTGTGCCTGGAAGATTGGG
CTGCCGAGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT211155 WP_000781370.1 NZ_CP080163:3234267-3234551 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT211155 NZ_CP104727:c1002041-1001781 [Pseudomonas citronellolis]
ATGTCCTCGCCCACACTCCCCAGCCGCGTCTTCATGAACGGCAACAGCCAGGCGGTGCGCATTCCCCAGGAGTTCCGCCT
GGACGCCAGCCGCGTGGAGATCCGCCGCCAAGCCAATGGCGACCTGGTCATCCACCCATTGCCCGAGGACCGCGGCGAGG
CGTTGCTGCAGGCGCTGTCCGGCTTCGACGAGGCGTTCATCGAAGCGCTGGAGGAAGGGCAGCGCGAACAGCCGCCGATG
CAGGAGCGGGAAGAGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References