Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1280126..1280925 Replicon chromosome
Accession NZ_CP080163
Organism Escherichia coli strain PK8275

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag KZY08_RS06210 Protein ID WP_000347269.1
Coordinates 1280126..1280590 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag KZY08_RS06215 Protein ID WP_001309780.1
Coordinates 1280590..1280925 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY08_RS06180 (1275127) 1275127..1275561 - 435 WP_000948818.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KZY08_RS06185 (1275579) 1275579..1276457 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KZY08_RS06190 (1276447) 1276447..1277226 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZY08_RS06195 (1277237) 1277237..1277710 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZY08_RS06200 (1277733) 1277733..1279013 - 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZY08_RS06205 (1279262) 1279262..1280071 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
KZY08_RS06210 (1280126) 1280126..1280590 - 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZY08_RS06215 (1280590) 1280590..1280925 - 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZY08_RS06220 (1281074) 1281074..1282645 - 1572 WP_001273738.1 galactarate dehydratase -
KZY08_RS06225 (1283020) 1283020..1284354 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KZY08_RS06230 (1284370) 1284370..1285140 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T211140 WP_000347269.1 NZ_CP080163:c1280590-1280126 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T211140 NZ_CP104721:3534119-3534337 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT211140 WP_001309780.1 NZ_CP080163:c1280925-1280590 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT211140 NZ_CP104721:3533719-3534093 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References