Detailed information of TA system TA211127

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 128517..128938
• Replicon: plasmid pPK8261-180kb
• Accession: NZ_CP080157
• Organism: Escherichia coli strain PK8261

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KZY07_RS26350
• Protein ID: WP_096937776.1
• Coordinates: 128517..128642 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	128740..128938 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY07_RS26315 (123555)	123555..123770	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
KZY07_RS26320 (123906)	123906..124553	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
KZY07_RS26325 (124745)	124745..125128	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KZY07_RS26330 (125468)	125468..126070	+	603	WP_077248356.1	transglycosylase SLT domain-containing protein	-
KZY07_RS26335 (126365)	126365..127186	-	822	WP_001234475.1	DUF932 domain-containing protein	-
KZY07_RS26340 (127305)	127305..127592	-	288	WP_000107546.1	hypothetical protein	-
KZY07_RS26345 (127617)	127617..127823	-	207	WP_000547965.1	hypothetical protein	-
KZY07_RS27940 (127893)	127893..128066	+	174	Protein_140	hypothetical protein	-
KZY07_RS27945 (128064)	128064..128294	-	231	WP_232380134.1	hypothetical protein	-
KZY07_RS26350 (128517)	128517..128642	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
KZY07_RS27950 (128584)	128584..128733	-	150	Protein_143	DUF5431 family protein	-
- (128740)	128740..128938	-	199	NuclAT_0	-	Antitoxin
- (128740)	128740..128938	-	199	NuclAT_0	-	Antitoxin
- (128740)	128740..128938	-	199	NuclAT_0	-	Antitoxin
- (128740)	128740..128938	-	199	NuclAT_0	-	Antitoxin
KZY07_RS26355 (128907)	128907..129669	-	763	Protein_144	plasmid SOS inhibition protein A	-
KZY07_RS26360 (129666)	129666..130100	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
KZY07_RS26365 (130155)	130155..132113	-	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
KZY07_RS26370 (132179)	132179..132412	-	234	WP_000005990.1	DUF905 family protein	-
KZY07_RS26375 (132469)	132469..133008	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
KZY07_RS26380 (133326)	133326..133835	+	510	WP_071600123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / dfrA14 / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..180318	180318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T211127 WP_096937776.1 NZ_CP080157:c128642-128517 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T211127 NZ_CP104721:c611910-611446 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT211127 NZ_CP080157:c128938-128740 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin