Detailed information of TA system TA211124

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90336..90590
• Replicon: plasmid pPK8261-180kb
• Accession: NZ_CP080157
• Organism: Escherichia coli strain PK8261

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KZY07_RS26125
• Protein ID: WP_001312851.1
• Coordinates: 90336..90485 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	90529..90590 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY07_RS26100 (85618)	85618..88623	+	3006	WP_001143760.1	Tn3-like element Tn3 family transposase	-
KZY07_RS26105 (88556)	88556..89021	-	466	Protein_90	plasmid replication initiator RepA	-
KZY07_RS26110 (89014)	89014..89496	-	483	WP_001273588.1	hypothetical protein	-
KZY07_RS27930 (89489)	89489..89536	-	48	WP_229471593.1	hypothetical protein	-
KZY07_RS27935 (89527)	89527..89778	+	252	WP_223195197.1	replication protein RepA	-
KZY07_RS26120 (89795)	89795..90052	-	258	WP_000083845.1	replication regulatory protein RepA	-
KZY07_RS26125 (90336)	90336..90485	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (90529)	90529..90590	+	62	NuclAT_1	-	Antitoxin
- (90529)	90529..90590	+	62	NuclAT_1	-	Antitoxin
- (90529)	90529..90590	+	62	NuclAT_1	-	Antitoxin
- (90529)	90529..90590	+	62	NuclAT_1	-	Antitoxin
KZY07_RS26130 (90846)	90846..90920	-	75	Protein_96	endonuclease	-
KZY07_RS26135 (91166)	91166..91378	-	213	WP_001299730.1	ANR family transcriptional regulator	-
KZY07_RS26140 (91514)	91514..92074	-	561	WP_000139315.1	fertility inhibition protein FinO	-
KZY07_RS26145 (92177)	92177..93037	-	861	WP_000704513.1	alpha/beta hydrolase	-
KZY07_RS26150 (93096)	93096..93842	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / dfrA14 / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..180318	180318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T211124 WP_001312851.1 NZ_CP080157:c90485-90336 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T211124 NZ_CP104720:c4437945-4437580 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT211124 NZ_CP080157:90529-90590 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin