Detailed information of TA system TA211122

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4801..5070
• Replicon: plasmid pPK8261-tetX
• Accession: NZ_CP080156
• Organism: Escherichia coli strain PK8261

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KZY07_RS25000
• Protein ID: WP_001372321.1
• Coordinates: 4945..5070 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	4801..4866 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY07_RS24965 (336)	336..542	+	207	WP_000275858.1	hypothetical protein	-
KZY07_RS24970 (568)	568..1107	+	540	WP_001825195.1	single-stranded DNA-binding protein	-
KZY07_RS24975 (1170)	1170..1403	+	234	WP_000005990.1	DUF905 family protein	-
KZY07_RS24980 (1469)	1469..3427	+	1959	WP_001825193.1	ParB/RepB/Spo0J family partition protein	-
KZY07_RS24985 (3482)	3482..3916	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KZY07_RS24990 (3913)	3913..4675	+	763	Protein_6	plasmid SOS inhibition protein A	-
KZY07_RS24995 (4644)	4644..4832	-	189	WP_001299721.1	hypothetical protein	-
- (4801)	4801..4866	+	66	NuclAT_1	-	-
- (4801)	4801..4866	-	66	NuclAT_0	-	Antitoxin
- (4801)	4801..4866	-	66	NuclAT_0	-	Antitoxin
- (4644)	4644..4868	+	225	NuclAT_0	-	-
- (4644)	4644..4868	+	225	NuclAT_0	-	-
- (4644)	4644..4868	+	225	NuclAT_0	-	-
- (4644)	4644..4868	+	225	NuclAT_0	-	-
- (4644)	4644..4868	-	225	NuclAT_0	-	-
KZY07_RS27855 (4854)	4854..5003	+	150	Protein_8	plasmid maintenance protein Mok	-
KZY07_RS25000 (4945)	4945..5070	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KZY07_RS27860 (5290)	5290..5520	+	231	WP_001426396.1	hypothetical protein	-
KZY07_RS27865 (5518)	5518..5691	-	174	Protein_11	hypothetical protein	-
KZY07_RS27870 (5761)	5761..5967	+	207	WP_000547968.1	hypothetical protein	-
KZY07_RS25005 (5992)	5992..6279	+	288	WP_000107544.1	hypothetical protein	-
KZY07_RS25010 (6400)	6400..7221	+	822	WP_001234469.1	DUF932 domain-containing protein	-
KZY07_RS25015 (7518)	7518..8165	-	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
KZY07_RS25020 (8442)	8442..8825	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KZY07_RS25025 (9016)	9016..9702	+	687	WP_000332484.1	PAS domain-containing protein	-
KZY07_RS25030 (9796)	9796..10023	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T211122 WP_001372321.1 NZ_CP080156:4945-5070 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T211122 NZ_CP104720:3254478-3255056 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGTTCGGCGCGACGCCTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT211122 NZ_CP080156:c4866-4801 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin