Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2625346..2625908 Replicon chromosome
Accession NZ_CP080155
Organism Escherichia coli strain PK8261

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag KZY07_RS12625 Protein ID WP_000605676.1
Coordinates 2625346..2625624 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag KZY07_RS12630 Protein ID WP_000781370.1
Coordinates 2625624..2625908 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY07_RS12600 (2620932) 2620932..2621363 - 432 WP_000152305.1 peroxiredoxin OsmC -
KZY07_RS12605 (2621708) 2621708..2621923 + 216 WP_000495766.1 biofilm-dependent modulation protein -
KZY07_RS12610 (2622025) 2622025..2622162 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
KZY07_RS12615 (2622319) 2622319..2624016 + 1698 WP_000433464.1 malate dehydrogenase -
KZY07_RS12620 (2624150) 2624150..2625160 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
KZY07_RS12625 (2625346) 2625346..2625624 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZY07_RS12630 (2625624) 2625624..2625908 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
KZY07_RS12635 (2626136) 2626136..2626789 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
KZY07_RS12640 (2626782) 2626782..2627666 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
KZY07_RS12645 (2627679) 2627679..2630726 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T211111 WP_000605676.1 NZ_CP080155:2625346-2625624 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T211111 NZ_CP104691:3181274-3181684 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCCTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGCGCGCCGTGGATGCTTAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT211111 WP_000781370.1 NZ_CP080155:2625624-2625908 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT211111 NZ_CP104691:3181027-3181293 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References