Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 670708..671507 Replicon chromosome
Accession NZ_CP080155
Organism Escherichia coli strain PK8261

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag KZY07_RS03340 Protein ID WP_000347269.1
Coordinates 670708..671172 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag KZY07_RS03345 Protein ID WP_001309780.1
Coordinates 671172..671507 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY07_RS03310 (665709) 665709..666143 - 435 WP_000948818.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KZY07_RS03315 (666161) 666161..667039 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KZY07_RS03320 (667029) 667029..667808 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZY07_RS03325 (667819) 667819..668292 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZY07_RS03330 (668315) 668315..669595 - 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZY07_RS03335 (669844) 669844..670653 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
KZY07_RS03340 (670708) 670708..671172 - 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZY07_RS03345 (671172) 671172..671507 - 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZY07_RS03350 (671656) 671656..673227 - 1572 WP_001273738.1 galactarate dehydratase -
KZY07_RS03355 (673602) 673602..674936 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KZY07_RS03360 (674952) 674952..675722 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T211096 WP_000347269.1 NZ_CP080155:c671172-670708 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T211096 NZ_CP104681:c4234739-4234464 [Escherichia coli]
ATGGAAATTTACTGGACGTTTAAAGCTCAGGATGACCTAGAACGTATCTATCGTTTCGCCCTTCAGTATAGCCGCAAGCA
CGCCGATGACATTTTGGATCGCCTAATTACTGGCTGCGCTGGCCTTACAGCGCATCCGGCAATTGGTGTACATCAGACTC
GTTATGAACCCCGAGAAGTCCGAAAAGTGTTATTTGATGACTATGAGGTTCACTACGAAATCCGTGACAATGATATCCAT
ATCGTGGATCTCTGGCATACAAAAGAAGAACGATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT211096 WP_001309780.1 NZ_CP080155:c671507-671172 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT211096 NZ_CP104681:c4234991-4234743 [Escherichia coli]
ATGGGCTCACTGACTAAACAAACCATCAGCGCACAGATACCCGTTGAGTTGGCTGCTGCTGTTGAGAATCTGGCAATTGA
GTTAGATCGCTCTAAAAGCTGGATTATTAAGGAAGCGTTGACCTGTATGCTGGCCGAAAGAGAGCGGCGTCACCAGAGCA
TCCAGGCCGGTCTTGCCGATGTGGATGCGGGTCGCGTGGTGAGCCACTTAGACATGGTGGATTTTGCTAATCGCTTAAAG
AAAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References