Detailed information of TA system TA211092

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72211..72637
• Replicon: plasmid pPK8276-100kb
• Accession: NZ_CP080151
• Organism: Escherichia coli strain PK8276

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KZY06_RS25895
• Protein ID: WP_001372321.1
• Coordinates: 72211..72336 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	72413..72637 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY06_RS25855 (67257)	67257..67484	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
KZY06_RS25860 (67578)	67578..68264	-	687	WP_000332484.1	PAS domain-containing protein	-
KZY06_RS25865 (68455)	68455..68838	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KZY06_RS25870 (69115)	69115..69762	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
KZY06_RS25875 (70059)	70059..70880	-	822	WP_001234469.1	DUF932 domain-containing protein	-
KZY06_RS25880 (71002)	71002..71289	-	288	WP_000107535.1	hypothetical protein	-
KZY06_RS25885 (71314)	71314..71520	-	207	WP_000547939.1	hypothetical protein	-
KZY06_RS26530 (71590)	71590..71763	+	174	Protein_87	hypothetical protein	-
KZY06_RS26535 (71761)	71761..71991	-	231	WP_001426396.1	hypothetical protein	-
KZY06_RS25895 (72211)	72211..72336	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KZY06_RS26540 (72278)	72278..72427	-	150	Protein_90	plasmid maintenance protein Mok	-
- (72413)	72413..72637	-	225	NuclAT_0	-	Antitoxin
- (72413)	72413..72637	-	225	NuclAT_0	-	Antitoxin
- (72413)	72413..72637	-	225	NuclAT_0	-	Antitoxin
- (72413)	72413..72637	-	225	NuclAT_0	-	Antitoxin
KZY06_RS25900 (72449)	72449..72637	+	189	WP_001299721.1	hypothetical protein	-
KZY06_RS25905 (72606)	72606..73368	-	763	Protein_92	plasmid SOS inhibition protein A	-
KZY06_RS25910 (73365)	73365..73799	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
KZY06_RS25915 (73854)	73854..75812	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
KZY06_RS25920 (75871)	75871..76104	-	234	WP_000006004.1	DUF905 domain-containing protein	-
KZY06_RS25925 (76160)	76160..76687	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
KZY06_RS25930 (77160)	77160..77414	-	255	WP_072163210.1	hypothetical protein	-
KZY06_RS26545 (77330)	77330..77566	-	237	WP_077816166.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / aph(3')-Ia / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / sitABCD	-	1..100987	100987

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T211092 WP_001372321.1 NZ_CP080151:c72336-72211 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T211092 NZ_CP104681:3791047-3791388 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT211092 NZ_CP080151:c72637-72413 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin