Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2840543..2841084 Replicon chromosome
Accession NZ_CP080146
Organism Escherichia coli strain PK8276

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag KZY06_RS13715 Protein ID WP_000615981.1
Coordinates 2840806..2841084 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KZY06_RS13710 Protein ID WP_000729704.1
Coordinates 2840543..2840803 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY06_RS13690 (2836226) 2836226..2837011 - 786 WP_086977314.1 putative lateral flagellar export/assembly protein LafU -
KZY06_RS13695 (2836983) 2836983..2838695 + 1713 Protein_2697 flagellar biosynthesis protein FlhA -
KZY06_RS13700 (2838911) 2838911..2839408 - 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
KZY06_RS13705 (2839584) 2839584..2840333 - 750 WP_086977304.1 C40 family peptidase -
KZY06_RS13710 (2840543) 2840543..2840803 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KZY06_RS13715 (2840806) 2840806..2841084 + 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KZY06_RS13720 (2841240) 2841240..2841980 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KZY06_RS13725 (2841951) 2841951..2842718 - 768 WP_000333379.1 class II glutamine amidotransferase -
KZY06_RS13730 (2842924) 2842924..2843502 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T211074 WP_000615981.1 NZ_CP080146:2840806-2841084 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T211074 NZ_CP104679:71189-71500 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT211074 WP_000729704.1 NZ_CP080146:2840543-2840803 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT211074 NZ_CP104679:71497-71916 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References