Detailed information of TA system TA211053

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40006..40260
• Replicon: plasmid pPK8241-82kb
• Accession: NZ_CP080142
• Organism: Escherichia coli strain PK8241

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KZY05_RS28730
• Protein ID: WP_001312851.1
• Coordinates: 40111..40260 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	40006..40067 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY05_RS28715 (37959)	37959..38702	+	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-
KZY05_RS28720 (38757)	38757..39317	+	561	WP_000139310.1	fertility inhibition protein FinO	-
KZY05_RS28725 (39452)	39452..39664	+	213	WP_001541896.1	ANR family transcriptional regulator	-
- (40006)	40006..40067	-	62	NuclAT_1	-	Antitoxin
- (40006)	40006..40067	-	62	NuclAT_1	-	Antitoxin
- (40006)	40006..40067	-	62	NuclAT_1	-	Antitoxin
- (40006)	40006..40067	-	62	NuclAT_1	-	Antitoxin
KZY05_RS28730 (40111)	40111..40260	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
KZY05_RS28735 (40528)	40528..40785	+	258	WP_001541895.1	replication regulatory protein RepA	-
KZY05_RS28740 (41017)	41017..41091	+	75	WP_061357229.1	RepA leader peptide Tap	-
KZY05_RS28745 (41084)	41084..41941	+	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
KZY05_RS28750 (42907)	42907..43158	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KZY05_RS28755 (43155)	43155..43442	+	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KZY05_RS28760 (43517)	43517..43705	-	189	Protein_55	IS3 family transposase	-
KZY05_RS28765 (43739)	43739..44239	-	501	Protein_56	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / tet(A) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M)	-	1..82038	82038

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T211053 WP_001312851.1 NZ_CP080142:40111-40260 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T211053 NZ_CP104677:c956752-956225 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGAAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT211053 NZ_CP080142:c40067-40006 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin