Detailed information of TA system TA211050

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4692..4961
• Replicon: plasmid pPK8241-82kb
• Accession: NZ_CP080142
• Organism: Escherichia coli strain PK8241

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KZY05_RS28545
• Protein ID: WP_001372321.1
• Coordinates: 4836..4961 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	4692..4757 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY05_RS29660	3..164	-	162	Protein_1	hypothetical protein	-
KZY05_RS28510	234..440	+	207	WP_000275853.1	hypothetical protein	-
KZY05_RS28515	466..1005	+	540	WP_032332913.1	single-stranded DNA-binding protein	-
KZY05_RS28520	1062..1295	+	234	WP_000005987.1	DUF905 family protein	-
KZY05_RS28525	1360..3318	+	1959	WP_039000998.1	ParB/RepB/Spo0J family partition protein	-
KZY05_RS28530	3373..3807	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KZY05_RS28535	3804..4566	+	763	Protein_7	plasmid SOS inhibition protein A	-
KZY05_RS28540	4535..4723	-	189	WP_001299721.1	hypothetical protein	-
-	4535..4759	+	225	NuclAT_0	-	-
-	4535..4759	+	225	NuclAT_0	-	-
-	4535..4759	+	225	NuclAT_0	-	-
-	4535..4759	+	225	NuclAT_0	-	-
-	4692..4757	-	66	-	-	Antitoxin
KZY05_RS29665	4745..4894	+	150	Protein_9	plasmid maintenance protein Mok	-
KZY05_RS28545	4836..4961	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KZY05_RS28550	5181..5411	+	231	WP_001426396.1	hypothetical protein	-
KZY05_RS29670	5409..5582	-	174	Protein_12	hypothetical protein	-
KZY05_RS29675	5652..5858	+	207	WP_000547968.1	hypothetical protein	-
KZY05_RS28555	5883..6170	+	288	WP_000107535.1	hypothetical protein	-
KZY05_RS28560	6292..7113	+	822	WP_072795862.1	DUF932 domain-containing protein	-
KZY05_RS28565	7410..8012	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
KZY05_RS28570	8333..8716	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KZY05_RS28575	8903..9592	+	690	WP_039002187.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / tet(A) / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M)	-	1..82038	82038

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T211050 WP_001372321.1 NZ_CP080142:4836-4961 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T211050 NZ_CP104677:286361-286729 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTATCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCACTATATCCCGTGGGTATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAACAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT211050 NZ_CP080142:c4757-4692 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin