Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 5086438..5087132 | Replicon | chromosome |
| Accession | NZ_CP080139 | ||
| Organism | Escherichia coli strain PK8241 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | KZY05_RS25625 | Protein ID | WP_001263493.1 |
| Coordinates | 5086734..5087132 (+) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | KZY05_RS25620 | Protein ID | WP_000554757.1 |
| Coordinates | 5086438..5086731 (+) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KZY05_RS25600 (5082070) | 5082070..5082567 | + | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
| KZY05_RS25605 (5082791) | 5082791..5084503 | - | 1713 | Protein_5050 | flagellar biosynthesis protein FlhA | - |
| KZY05_RS25610 (5084475) | 5084475..5085260 | + | 786 | WP_000207587.1 | putative lateral flagellar export/assembly protein LafU | - |
| KZY05_RS25615 (5085331) | 5085331..5086386 | + | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| KZY05_RS25620 (5086438) | 5086438..5086731 | + | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| KZY05_RS25625 (5086734) | 5086734..5087132 | + | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| KZY05_RS25630 (5087142) | 5087142..5087594 | + | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| KZY05_RS25635 (5087840) | 5087840..5088043 | + | 204 | Protein_5056 | RtcB family protein | - |
| KZY05_RS25640 (5088042) | 5088042..5088563 | + | 522 | Protein_5057 | peptide chain release factor H | - |
| KZY05_RS25645 (5088620) | 5088620..5090077 | - | 1458 | WP_001293003.1 | cytosol nonspecific dipeptidase | - |
| KZY05_RS25650 (5090338) | 5090338..5090796 | + | 459 | WP_001291994.1 | xanthine phosphoribosyltransferase | - |
| - (5091392) | 5091392..5091472 | + | 81 | NuclAT_11 | - | - |
| - (5091392) | 5091392..5091472 | + | 81 | NuclAT_11 | - | - |
| - (5091392) | 5091392..5091472 | + | 81 | NuclAT_11 | - | - |
| - (5091392) | 5091392..5091472 | + | 81 | NuclAT_11 | - | - |
| KZY05_RS25655 (5090888) | 5090888..5092132 | + | 1245 | WP_000189541.1 | esterase FrsA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T211045 WP_001263493.1 NZ_CP080139:5086734-5087132 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T211045 NZ_CP104666:c130674-130144 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT211045 WP_000554757.1 NZ_CP080139:5086438-5086731 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT211045 NZ_CP104666:c130947-130678 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|