Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3193660..3194387 Replicon chromosome
Accession NZ_CP080139
Organism Escherichia coli strain PK8241

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KZY05_RS16285 Protein ID WP_000550189.1
Coordinates 3194073..3194387 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KZY05_RS16280 Protein ID WP_000560255.1
Coordinates 3193660..3194076 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY05_RS16270 (3188820) 3188820..3191171 + 2352 WP_000695486.1 alpha-glucosidase -
KZY05_RS16275 (3191597) 3191597..3193615 + 2019 WP_219788350.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KZY05_RS16280 (3193660) 3193660..3194076 - 417 WP_000560255.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KZY05_RS16285 (3194073) 3194073..3194387 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KZY05_RS16290 (3194671) 3194671..3195807 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KZY05_RS16295 (3195892) 3195892..3196395 + 504 WP_001300832.1 M48 family metallopeptidase -
KZY05_RS16300 (3196472) 3196472..3197164 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KZY05_RS16305 (3197243) 3197243..3198229 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T211036 WP_000550189.1 NZ_CP080139:c3194387-3194073 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T211036 NZ_CP104665:3775536-3775754 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14951.41 Da        Isoelectric Point: 4.5577

>AT211036 WP_000560255.1 NZ_CP080139:c3194076-3193660 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT211036 NZ_CP104665:3775136-3775510 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References