Detailed information of TA system TA211009

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 61127..61396
• Replicon: plasmid pPK8277-tetX
• Accession: NZ_CP080134
• Organism: Escherichia coli strain PK8277

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KZY04_RS22660
• Protein ID: WP_001372321.1
• Coordinates: 61271..61396 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	61127..61192 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY04_RS24295 (56155)	56155..56421	+	267	WP_072667006.1	hypothetical protein	-
KZY04_RS22625 (56662)	56662..56868	+	207	WP_000275858.1	hypothetical protein	-
KZY04_RS22630 (56894)	56894..57433	+	540	WP_001825195.1	single-stranded DNA-binding protein	-
KZY04_RS22635 (57496)	57496..57729	+	234	WP_000005990.1	DUF905 family protein	-
KZY04_RS22640 (57795)	57795..59753	+	1959	WP_001825193.1	ParB/RepB/Spo0J family partition protein	-
KZY04_RS22645 (59808)	59808..60242	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KZY04_RS22650 (60239)	60239..61001	+	763	Protein_72	plasmid SOS inhibition protein A	-
KZY04_RS22655 (60970)	60970..61158	-	189	WP_001299721.1	hypothetical protein	-
- (61127)	61127..61192	+	66	NuclAT_1	-	-
- (61127)	61127..61192	-	66	NuclAT_0	-	Antitoxin
- (61127)	61127..61192	-	66	NuclAT_0	-	Antitoxin
- (60970)	60970..61194	+	225	NuclAT_0	-	-
- (60970)	60970..61194	+	225	NuclAT_0	-	-
- (60970)	60970..61194	+	225	NuclAT_0	-	-
- (60970)	60970..61194	+	225	NuclAT_0	-	-
- (60970)	60970..61194	-	225	NuclAT_0	-	-
KZY04_RS24300 (61180)	61180..61329	+	150	Protein_74	plasmid maintenance protein Mok	-
KZY04_RS22660 (61271)	61271..61396	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KZY04_RS24305 (61616)	61616..61846	+	231	WP_001426396.1	hypothetical protein	-
KZY04_RS24310 (61844)	61844..62017	-	174	Protein_77	hypothetical protein	-
KZY04_RS24315 (62087)	62087..62293	+	207	WP_000547968.1	hypothetical protein	-
KZY04_RS22665 (62318)	62318..62605	+	288	WP_000107544.1	hypothetical protein	-
KZY04_RS22670 (62726)	62726..63547	+	822	WP_001234469.1	DUF932 domain-containing protein	-
KZY04_RS22675 (63844)	63844..64491	-	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
KZY04_RS22680 (64768)	64768..65151	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KZY04_RS22685 (65342)	65342..66028	+	687	WP_000332484.1	PAS domain-containing protein	-
KZY04_RS22690 (66122)	66122..66349	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T211009 WP_001372321.1 NZ_CP080134:61271-61396 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T211009 NZ_CP104660:711526-712011 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTAATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT211009 NZ_CP080134:c61192-61127 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin