211008

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4135025..4135522 Replicon chromosome
Accession NZ_CP080133
Organism Escherichia coli strain PK8277

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KZY04_RS20070 Protein ID WP_001173343.1
Coordinates 4135025..4135198 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KZY04_RS20075 Protein ID WP_000398619.1
Coordinates 4135226..4135522 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY04_RS20060 (4131690) 4131690..4133147 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KZY04_RS20065 (4133384) 4133384..4134901 + 1518 WP_001295074.1 lysine--tRNA ligase -
KZY04_RS20070 (4135025) 4135025..4135198 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KZY04_RS20075 (4135226) 4135226..4135522 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KZY04_RS20080 (4135749) 4135749..4136021 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KZY04_RS20085 (4136033) 4136033..4136263 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KZY04_RS20090 (4136444) 4136444..4138075 + 1632 WP_001565376.1 sensor histidine kinase -
KZY04_RS20095 (4138072) 4138072..4138791 + 720 WP_219795029.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4126167..4135522 9355


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T211008 WP_001173343.1 NZ_CP080133:c4135198-4135025 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T211008 NZ_CP104660:328836-329204 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT211008 WP_000398619.1 NZ_CP080133:c4135522-4135226 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT211008 NZ_CP104660:328618-328839 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References