Detailed information of TA system TA211007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3629594..3630288
• Replicon: chromosome
• Accession: NZ_CP080133
• Organism: Escherichia coli strain PK8277

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KZY04_RS17675
• Protein ID: WP_001263493.1
• Coordinates: 3629594..3629992 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KZY04_RS17680
• Protein ID: WP_000554757.1
• Coordinates: 3629995..3630288 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3625254)	3625254..3625334	-	81	NuclAT_8	-	-
- (3625254)	3625254..3625334	-	81	NuclAT_8	-	-
- (3625254)	3625254..3625334	-	81	NuclAT_8	-	-
- (3625254)	3625254..3625334	-	81	NuclAT_8	-	-
KZY04_RS17645 (3624594)	3624594..3625838	-	1245	WP_000189541.1	esterase FrsA	-
KZY04_RS17650 (3625930)	3625930..3626388	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KZY04_RS17655 (3626649)	3626649..3628106	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
KZY04_RS17660 (3628163)	3628163..3628684	-	522	Protein_3484	peptide chain release factor H	-
KZY04_RS17665 (3628683)	3628683..3628886	-	204	Protein_3485	RtcB family protein	-
KZY04_RS17670 (3629132)	3629132..3629584	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KZY04_RS17675 (3629594)	3629594..3629992	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KZY04_RS17680 (3629995)	3629995..3630288	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KZY04_RS17685 (3630340)	3630340..3631395	-	1056	WP_001226164.1	DNA polymerase IV	-
KZY04_RS17690 (3631466)	3631466..3632251	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
KZY04_RS17695 (3632223)	3632223..3633935	+	1713	Protein_3491	flagellar biosynthesis protein FlhA	-
KZY04_RS17700 (3634159)	3634159..3634656	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T211007 WP_001263493.1 NZ_CP080133:c3629992-3629594 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T211007 NZ_CP104659:c5286655-5286437 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT211007 WP_000554757.1 NZ_CP080133:c3630288-3629995 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT211007 NZ_CP104659:c5287056-5286682 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6