210991

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 649712..650439 Replicon chromosome
Accession NZ_CP080133
Organism Escherichia coli strain PK8277

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KZY04_RS03185 Protein ID WP_000550189.1
Coordinates 649712..650026 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KZY04_RS03190 Protein ID WP_000560266.1
Coordinates 650023..650439 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY04_RS03165 (645870) 645870..646856 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
KZY04_RS03170 (646935) 646935..647627 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KZY04_RS03175 (647704) 647704..648207 - 504 WP_001300832.1 M48 family metallopeptidase -
KZY04_RS03180 (648292) 648292..649428 + 1137 WP_000018683.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KZY04_RS03185 (649712) 649712..650026 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KZY04_RS03190 (650023) 650023..650439 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KZY04_RS03195 (650484) 650484..652502 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KZY04_RS03200 (652928) 652928..655279 - 2352 WP_000695486.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T210991 WP_000550189.1 NZ_CP080133:649712-650026 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T210991 NZ_CP104652:143788-144036 [Acinetobacter variabilis]
ATGGGTAAAACGGAAAAGTTATTGGAAAAATTTGGAAATTCAAAAAATACGTTTCCATATAAAGATTTAGTCGTTTTACT
GAGCCAGCTCGGCTATGAAAAATTTGAAATGGCAGGCTCTCGTGTTCGGTTTTTTAATGAAAAAACTGAACATATGATTT
TACTGCATAGACCACACCCTGAAAATGAAATCAAAGGTGGGGCATTGAAAGCTGTAAAACAGGCACTCAAGCAGGAGGGA
TTTTTATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT210991 WP_000560266.1 NZ_CP080133:650023-650439 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT210991 NZ_CP104652:144033-144365 [Acinetobacter variabilis]
ATGAGCTATTTAAAATACAAAGGTTATCTCGGAACAATTGAGCCTGATCTTGAAACAGGTGAGTTATTTGGAAAACTTGC
TTTTATTCGTGATCTTATCACCTATGAAGCGGAAACTTTAAAAGCCCTTGAGAAAGCTTTTCAGGAATCTGTAGACGGCT
ATTTAGAATCATGTGCTGAACTAGGAAAATCTCCTGATCAACCATTCAAAGGCACTTTTAATGTCCGTATTAGCCCTGAA
TTGCACCGTAAGGCGGTTTTAGCATCAAGTAATTCTCTAAATGCCTTTGTCAGTGATGCGATTCAAGAAAAGTTAATGCG
TTTAGGTGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References