Detailed information of TA system TA210985

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 48829..49098
• Replicon: plasmid pPK8568-tetX
• Accession: NZ_CP080129
• Organism: Escherichia coli strain PK8568

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KZY03_RS24355
• Protein ID: WP_001372321.1
• Coordinates: 48973..49098 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	48829..48894 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY03_RS24315	43845..44123	+	279	WP_072127934.1	hypothetical protein	-
KZY03_RS24320	44364..44570	+	207	WP_000275858.1	hypothetical protein	-
KZY03_RS24325	44596..45135	+	540	WP_001825195.1	single-stranded DNA-binding protein	-
KZY03_RS24330	45198..45431	+	234	WP_000005990.1	DUF905 family protein	-
KZY03_RS24335	45497..47455	+	1959	WP_001825193.1	ParB/RepB/Spo0J family partition protein	-
KZY03_RS24340	47510..47944	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KZY03_RS24345	47941..48703	+	763	Protein_58	plasmid SOS inhibition protein A	-
KZY03_RS24350	48672..48860	-	189	WP_001299721.1	hypothetical protein	-
-	48672..48896	+	225	NuclAT_0	-	-
-	48672..48896	+	225	NuclAT_0	-	-
-	48672..48896	+	225	NuclAT_0	-	-
-	48672..48896	+	225	NuclAT_0	-	-
-	48829..48894	-	66	-	-	Antitoxin
KZY03_RS25190	48882..49031	+	150	Protein_60	plasmid maintenance protein Mok	-
KZY03_RS24355	48973..49098	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KZY03_RS25195	49318..49548	+	231	WP_001426396.1	hypothetical protein	-
KZY03_RS25200	49546..49719	-	174	Protein_63	hypothetical protein	-
KZY03_RS25205	49789..49995	+	207	WP_000547968.1	hypothetical protein	-
KZY03_RS24360	50020..50307	+	288	WP_000107544.1	hypothetical protein	-
KZY03_RS24365	50428..51249	+	822	WP_001234469.1	DUF932 domain-containing protein	-
KZY03_RS24370	51546..52193	-	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
KZY03_RS24375	52470..52853	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KZY03_RS24380	53044..53730	+	687	WP_000332484.1	PAS domain-containing protein	-
KZY03_RS24385	53824..54051	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(X4) / floR / blaTEM-1C	-	1..91844	91844

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T210985 WP_001372321.1 NZ_CP080129:48973-49098 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T210985 NZ_CP104649:c5284589-5284203 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT210985 NZ_CP080129:c48894-48829 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin