Detailed information of TA system TA210980

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 132187..132613
• Replicon: plasmid pPK8568-156kb
• Accession: NZ_CP080127
• Organism: Escherichia coli strain PK8568

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KZY03_RS23280
• Protein ID: WP_001372321.1
• Coordinates: 132187..132312 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	132389..132613 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY03_RS23265 (127689)	127689..128393	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KZY03_RS23270 (128458)	128458..128802	+	345	Protein_132	recombinase family protein	-
KZY03_RS23275 (128806)	128806..131772	+	2967	WP_001138014.1	Tn3-like element TnAs1 family transposase	-
KZY03_RS25130 (131749)	131749..131967	-	219	WP_071780187.1	hypothetical protein	-
KZY03_RS23280 (132187)	132187..132312	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KZY03_RS25135 (132254)	132254..132403	-	150	Protein_136	plasmid maintenance protein Mok	-
- (132389)	132389..132613	-	225	NuclAT_0	-	Antitoxin
- (132389)	132389..132613	-	225	NuclAT_0	-	Antitoxin
- (132389)	132389..132613	-	225	NuclAT_0	-	Antitoxin
- (132389)	132389..132613	-	225	NuclAT_0	-	Antitoxin
KZY03_RS23285 (132425)	132425..132613	+	189	WP_001299721.1	hypothetical protein	-
KZY03_RS23290 (132582)	132582..133344	-	763	Protein_138	plasmid SOS inhibition protein A	-
KZY03_RS23295 (133341)	133341..133775	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
KZY03_RS23300 (133830)	133830..135788	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
KZY03_RS23305 (135847)	135847..136080	-	234	WP_000006004.1	DUF905 domain-containing protein	-
KZY03_RS23310 (136136)	136136..136663	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
KZY03_RS25140 (136982)	136982..137235	+	254	Protein_143	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / blaTEM-1C / dfrA14 / sitABCD	iroB / iroC / iroD / iroE / iroN	1..156326	156326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T210980 WP_001372321.1 NZ_CP080127:c132312-132187 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T210980 NZ_CP080127:c132312-132187 [Escherichia coli]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT210980 NZ_CP080127:c132613-132389 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin