Detailed information of TA system TA210977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4489257..4489859
• Replicon: chromosome
• Accession: NZ_CP080126
• Organism: Escherichia coli strain PK8568

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KZY03_RS21645
• Protein ID: WP_000897305.1
• Coordinates: 4489548..4489859 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZY03_RS21640
• Protein ID: WP_000356397.1
• Coordinates: 4489257..4489547 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY03_RS21615 (4484804)	4484804..4485439	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KZY03_RS21620 (4485436)	4485436..4486365	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KZY03_RS21625 (4486764)	4486764..4487744	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
KZY03_RS21630 (4487973)	4487973..4488215	-	243	WP_001086388.1	protein YiiF	-
KZY03_RS21635 (4488434)	4488434..4488652	-	219	WP_001295676.1	CopG family transcriptional regulator	-
KZY03_RS21640 (4489257)	4489257..4489547	-	291	WP_000356397.1	NadS family protein	Antitoxin
KZY03_RS21645 (4489548)	4489548..4489859	-	312	WP_000897305.1	hypothetical protein	Toxin
KZY03_RS21650 (4490088)	4490088..4490996	+	909	WP_001162704.1	alpha/beta hydrolase	-
KZY03_RS21655 (4491060)	4491060..4492001	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
KZY03_RS21660 (4492046)	4492046..4492483	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KZY03_RS21665 (4492480)	4492480..4493352	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KZY03_RS21670 (4493346)	4493346..4493945	-	600	WP_001295269.1	glucose-1-phosphatase	-
KZY03_RS21675 (4494044)	4494044..4494829	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4486764..4487744	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T210977 WP_000897305.1 NZ_CP080126:c4489859-4489548 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T210977 NZ_CP104649:c3169140-3168865 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT210977 WP_000356397.1 NZ_CP080126:c4489547-4489257 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT210977 NZ_CP104649:c3168868-3168509 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7